Table 1 Differentially expressed proteins identified from cellular extracts of axenic cultures of M. aeruginosa after tryptic digestion and MALDI-TOF/TOF analysis
From: Comparative proteomics between natural Microcystis isolates with a focus on microcystin synthesis
Spot n. | Sequence | Accession no | Species | Protein ID | Sequence coverage (%) | Mowse score | Function | Theoretical pI/Mw (kDa) | Experimental pI/Mw (kDa) |
|---|---|---|---|---|---|---|---|---|---|
m01 | KTFQGPPHGITVERD | 166367530 | M. aeruginosa | Ribulose bisophosphate carboxylase, major subunit | 5.0 | 82 | CO2 fixation (Calvin cycle) and photorespiration | 6.8 / 53.20 | 6.4 / 53.86 |
GIIIHIHR | |||||||||
m02 | KTFQGPPHGITVERD | 166367530 | M. aeruginosa | Ribulose bisophosphate carboxylase, major subunit | 9.0 | 92 | CO2 fixation (Calvin cycle) and photorespiration | 6.8 / 53.20 | 6.2 / 53.84 |
AVTMGFVDLMR | |||||||||
KTFEGPPHGIQAERD | |||||||||
m03 | KIAAEFPDNFRL | 166363998 | M. aeruginosa | Ferredoxin-NADP oxidoreductase | 5.5 | 44 | Oxidation and reduction (ficobilissome, thylakoid membrane) | 6.1 / 44.81 | 6.9 / 35.61 |
IAAEFPDNFR | |||||||||
m04 | KGILEYNDLPLVSSDYRG | 166365244 | M. aeruginosa | Glyceraldehyde-3-phosphate dehydrogenase | 5.3 | 101 | Glucose metabolic process (phosphorilation) | 6.3 / 36.64 | 6.6 / 38.49 |
m05 | RKPTGEILAISRI | 166365988 | M. aeruginosa | Fructose-1,6-bisphosphate aldolase class II (FBA) | 49 | 74 | Glycolysis | 6.0 / 39.15 | 5.6 / 43.01 |
YAGENFLRI | |||||||||
RKPTGEILAISR | |||||||||
KYAGENFLRH | |||||||||
m06 | KNIADHVAVEAMRE | 166365743 | M. aeruginosa | D-Frutose-1,6-bifosfatase classe II / sedoheptulose 1,7-bisfosfatase (FBPase classe 2/SBPase) | 10.2 | 78 | Carbohydrate biosynthesis (Calvin cycle) | 4.7 / 37.66 | 5.1 / 42.99 |
SIEELVVVVMDRPR | |||||||||
FFHGGAR | |||||||||
m07 | LQEMGITNPDR | 166365743 | M. aeruginosa | D-Frutose-1,6-bifosfatase classe II / sedoheptulose 1,7-bisfosfatase (FBPase classe 2/SBPase) | 6.7 | 59 | Carbohydrate biosynthesis (Calvin cycle) | 4.37 / 37.12 | 4.95 / 43.12 |
FVDTVHLFDQPK | |||||||||
m08 | RKPDFSAYIDPQRQ | 159027237 | M. aeruginosa | Phosphoribulokinase (PRK) | 18.0 | 107 | Carbohydrate metabolic process | 5.45 / 38.04 | 5.00 / 40.88 |
KVIVIEGLHPLYDERV | |||||||||
RKPDFSAYIDPQRQ | |||||||||
KVIVIEGLHPLYDERV | |||||||||
m09 | RKPDFSAYIDPQRQ | 159027237 | M. aeruginosa | Phosphoribulokinase (PRK) | 27.3 | 112 | Carbohydrate metabolic process | 5.45 / 38.03 | 4.86 / 40.77 |
KVIVIEGLHPLYDERV | |||||||||
RGHTYDDVMAAINSRK | |||||||||
RKPDFSAYIDPQRQ | |||||||||
KVIVIEGLHPLYDERV | |||||||||
VLSVLLGMTIGQIHR | |||||||||
m10 | TLPDLIHSAPR | 196257787 | Cyanothece sp. | FAD-dependent pyridine nucleotide-disulphide oxidoreductase | 2.6 | 25 | Oxidoreductase | 6.41 / 46.87 | 8.41 / 42.98 |
m11 | KTFQGPPHGITVERD | 166367530 | M. aeruginosa | ribulose bisophosphate carboxylase, major subunit | 10.6 | 91 | CO2 fixation (Calvin cycle) and photorespiration | 6.76 / 53.20 | 6.57 / 54.67 |
RGITMGFVDLMRE | |||||||||
KNHGIHFRV | |||||||||
TFQGPPHGITVER | |||||||||
m12 | RDNDVTIDDLFYRA | 166365540 | M. aeruginosa | Porin type major outer membrane protein | 15.7 | 106 | Transport (integral membrane protein) | 4.14 / 60.14 | 4.78 / 50.13 |
KFADINFVYVRS | |||||||||
RLQAGNFNNTFNQSGPTRT | |||||||||
KFADINFVYVRS | |||||||||
LQAGNFNNTFNQSGPTR | |||||||||
QSQTPAHQSRANQR | |||||||||
m13 | RDNDVTIDDLFYRA | 166365540 | M. aeruginosa | Porin type major outer membrane protein | 10.4 | 96 | Transport (integral membrane protein) | 4.14 / 60.14 | 4.64 / 50.1 |
LQAGNFNNTFNQSGPTR | |||||||||
RDVSPTAWAYEALRS | |||||||||
KFADINFVYVRS | |||||||||
m14 | LQAGNFNNTFNQSGPTR | 166365540 | M. aeruginosa | Porin type major outer membrane protein | 8.6 | 84 | Transport (integral membrane protein) | 4.14 / 60.14 | 4.48 / 50.43 |
KFADINFVYVRS | |||||||||
RLQAGNFNNTFNQSGPTRT | |||||||||
m15 | RGWINDINETQNTTVNYPIIADGDRK | 166366392 | M. aeruginosa | Peroxiredoxin | 19.4 | 180 | Cell redox homeostasis | 4.17/16.55 | 4.68 / 25.39 |
KVIALSVDSAESHRG | |||||||||
m16 | QYFGETDETVNLR | 166363870 | M. aeruginosa | Triosephosphate isomerase | 12.2 | 62 | Triosephosphate isomerase activity (glyconeogenesis and glycolysis) | 4.14 / 25.21 | 4.85 / 27.01 |
RQYFGETDETVNLRV | |||||||||
m17 | RPDYISDFLTK | 166368140 | M. aeruginosa | Superoxide dismutase (SOD) | 9.0 | 40 | Catalyzes of superoxide to peroxide and molecular oxygen | 5.83 / 21.72 | 4.6 / 21.50 |
YLDYQNR | |||||||||
m18 | IVQASTGLEVLSDSILVQKLR | 220908731 | Cyanothece sp. PCC 7425 | ABC transport related | 5.8 | 78 | Stress response | 6.07 / 64.52 | 4.99 / 18.61 |
FLIATPLLLALMR | |||||||||
m19 | RLITYGVVAGDVTPIEEIGLVGVRE | 166363768 | M. aeruginosa | Allophycocyanin alpha subunit | 25.5 | 104 | Photosynthesis (phycobilisome) | 4.21 / 17.19 | 3.79 / 18.09 |
YLSPGELDR | |||||||||
DMDYYLR | |||||||||
m20 | RVPAEIVFDQGLGDLFVCRV | 166364254 | M. aeruginosa | Carbonic anhydrase, beta-type (CA) | 8.3 | 130 | Carbon utilization | 8.45 / 26.10 | 7.50 / 21.93 |
m21 | KYAGENFLRH | 166365988 | M. aeruginosa | Fructose-1,6-bisphosphate aldolase class II (FBA) | 10.0 | 59 | Glycolysis | 6.00 / 39.16 | 5.39 / 43.1 |
RKPTGEILAISRI | |||||||||
RKPTGEILAISRI | |||||||||
m22 | RSIEELVVVVMDRPRH | 166365743 | M. aeruginosa | D-fructose 1,6- bisphosphatase class 2/sedoheptulose 1,7- bisphosphatase (FBPase classe 2/SBPase) | 14.2 | 67 | Carbohydrate biosynthesis (Calvin cycle) | 4.37 / 37.68 | 4.95 / 45.86 |
FFHGGAR | |||||||||
FVDTVHLFDQPK | |||||||||
RFVDTVHLFDQPKY | |||||||||
m23 | DVSPTAWAYEALR | 166365540 | M. aeruginosa | Porin type major outer membrane protein | 4.6 | 45 | Transport (integral membrane protein) | 4.14 / 60.14 | 3.94 / 57.46 |
DVERALTAPHLTR | |||||||||
m24 | RQYFGETDETVNLRV | 166363870 | M. aeruginosa | Triosephosphate isomerase | 6.5 | 62 | Triosephosphate isomerase activity (gluconeogenesis and glycolysis) | 4.14 / 25.21 | 4.85 / 27.01 |
RQYFGETDETVNLRV | |||||||||
m25 | RTFTEVAPQPAPEPSVSPIRG | 166367494 | M. aeruginosa | Hypothetical protein MAE_47530 | 30.8 | 69 | Non function related | 5.32 / 13.01 | 8.39 / 17.08 |
RGEPQLNPGDYVLGRV | |||||||||
m26 | RAPYDESEVVYHLDLYENKG | 159029497 | M. aeruginosa | Phycobilisome rod linker polypeptide | 2.1 | 119 | Photosynthesis (phycobilisome) | 9.24/ 32.21 | 5.51 / 29.88 |
FQQCLVQTR | |||||||||
RETPVMSQAEIHSR | |||||||||
m27 | LVSLGLLK | 159028959 | M. aeruginosa | Cobalt ABC transporter, inner membrane subunit CbiQ | 3.1 | 72 | Cobalt trasnport and biosynthetic process of cobalamin | 10.22/28.53 | 5.18 / 37.08 |
m28 | KKILLLLVLIVAVLNFGKT | 15642898 | T. maritima | Zinc ABC transporter, periplasmic zinc-binding protein | 7.1 | 61 | Metal transport | 6.25 / 30.12 | 4.85 / 52.85 |
m29 | KPDFSAYIDPQR | 166366586 | M. aeruginosa | Phosphoribulokinase (PRK) | 3.6 | 42 | Kinase and transferase | 5.12 / 37.85 | 4.42 / 36.73 |
ATP binding | |||||||||
m30 | QAGSIVCISSISGDR | 166367746 | M. aeruginosa | PHA-specific acetoacetyl-CoA reductase | 6.3 | 49 | Oxidoreductase | 5.66 / 25.18 | 5.89 / 27.19 |