Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots
- Lizhong He†1,
- Xiaomin Lu†1, 2,
- Jing Tian1,
- Yanjuan Yang1,
- Bin Li1,
- Jing Li1 and
- Shirong Guo1Email author
© He et al; licensee BioMed Central Ltd. 2012
Received: 19 January 2012
Accepted: 20 June 2012
Published: 12 July 2012
Hypoxia acts as a plant stress factor, particularly in cucumbers plants under hydroponic culture. Calcium is involved in stress signal transmission and in the growth of plants. To determine the effect of exogenous calcium on hypoxic-responsive proteins in cucumber (Cucumis sativus L. cv. Jinchun No.2) roots, proteomic analysis was performed using two-dimensional electrophoresis (2-DE) and mass spectrometry.
Cucumber roots were used to analyze the influence of hypoxia on plants. The expressions of 38 protein spots corresponding to enzymes were shown to change in response to hypoxia. Of these, 30 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS analysis). The proteins were categorized according to functional groups, including glycolysis, the tricarboxylic acid (TCA) cycle, fermentative metabolism, nitrogen metabolism, energy metabolism, protein synthesis and defense against stress. Exogenous calcium appeared to alleviate hypoxic stress via these metabolic and physiological systems. Western blotting was used to analyze the accumulation of alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC); calcium further increased the expression of ADH and PDC under hypoxia. In addition, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to assess the transcript levels of differentially expressed proteins.
Exogenous calcium enhanced the expression of enzymes involved in glycolysis, the TCA cycle, fermentative metabolism, nitrogen metabolism, and reactive oxygen species (ROS) defense in plants under hypoxia. Calcium appears to induce hypoxic tolerance of cucumber seedlings. These phenomena have prompted us to further investigate the mechanisms by which cucumbers respond to exogenous calcium under hypoxia.
KeywordsCucumber Calcium Hypoxic stress Proteomics
Cultivated plants that produce vegetables, crops and fruits are frequently subjected to submerged conditions (so-called hypoxia) caused by flooding , waterlogging , irrigation or hydroponic culture . Plants subjected to hypoxia undergo dramatic metabolic changes and induce defensive mechanisms to cope with the potential damage caused. Hypoxia induces enhanced aerenchyma formation, stem elongation, gas film around submerged-leaves  and shoot biomass . The first process to be influenced by the metabolic change induced by a shortage of oxygen is respiration. Hypoxic stress interferes with the electron transport system causing a lack of suitable electron acceptors, which appears to be linked to the saturated conditions of a redox state, accumulation of NAD(P)H and suppressed synthesis of ATP . Energy from respiratory metabolism is necessary for the growth and yield of plants. Cucumber plants are sensitive to hypoxia, which frequently causes large reductions in yield .
Exogenous calcium can improve the suppression of growth/development of plants and help to maintain cell function by relieving gene repression caused during salt stress , anoxia , and chilling . The involvement of calcium in oxygen debt responses is also observed in many plants. For example, the oxygen debt (anoxia) in cells of maize, rice and wheat plants causes a rise in cytoplasmic Ca2+ concentration [11, 12]. In addition, elevated calcium levels significantly influence metabolic fluxes and substrate oxidation under hypoxic condition . According to our previous research , exogenous calcium enhances the biomass and soluble protein content of cucumber seedlings under hypoxia (Additional file 1: Table S1). Thus, calcium appears to act as a signaling component during anoxia signal transduction in plants. The alteration in Ca2+ concentration seems to decrease cytosolic pH, which probably represents a major signal in cells under suspension culture and in intact seedlings . However, the mechanism of calcium’s involvement in resistance to hypoxia remains unclear.
Proteomic analysis, commonly using mass spectrometry (MS), is a powerful technique that facilitates the visualization and comparison of complex mixtures of proteins. Proteomic analysis provides a large amount of information on individual proteins involved in specific biological responses. Recently, there have been many proteomic studies of cucumber plants [7, 16–22]; however, none of them investigated the effects of exogenous calcium on the proteome of cucumber seedlings under root-room hypoxia stress. The purpose of the present study is to use proteomics to clarify the relationship between calcium and proteins in cucumber plants under hypoxic stress. We found that exogenous calcium could enhance both responsive metabolism and fermentative metabolism of cucumber seedlings, improving their tolerance to hypoxia. The results should provide a basis for future studies at both the physiological and molecular levels.
Results and discussion
Identification and functional classification of proteins by MALDI-TOF/TOF
Approximately 500 spots were detected in Coomassie blue (CBB)-stained gels. Thirty-eight of these spots showed significant changes in relative volume (>1.5-fold) and were excised from the gels for MALDI-TOF/TOF MS analyses.
Differentially expression proteins identified by MALDI-TOF/TOF MS
fprotein expression (%Vol)
A B C
JGCCJG2048B02.b Jatropha curcas L. germinating seeds (mixed stages) Jatropha curcas cDNA clone
Unnamed protein product
Aconitate hydratase, cytoplasmic
Citric acid cycle
Cucurbita maxima (winter squash)
V-type proton ATPase catalytic subunit A
Gossypium hirsutum (upland cotton)
Triticum aestivum (bread wheat)
Ricinus communis (castor bean)
starch synthase III
Triticum aestivum (bread wheat)
TransId-212581 CACATN1 Coffea arabica cDNA clone
CBOZ5962.b1 CBOZ Coccomyxa sp. C-169 8 kb Coccomyxa sp. C-169 cDNA clone
Coccomyxa subellipsoidea C-169
CLS_cLiFproots_25a3_1_h11cLibkit5LD_D06 CLS_cLiFproots_plant Festuca arundinacea cDNA clone
putative protein phosphatase 2 C
Oryza sativa Japonica Group
Glutamine synthetase cytosolic isozyme
Citric acid cycle
Malate dehydrogenase, cytoplasmic
Citric acid cycle
CLS_cLiFproots_52a4_1_b18cLibkit5LD_ A09 CLS_cLiFproots_plant Festuca arundinacea cDNA clone
Oryza sativa Japonica Group
putative pyruvate dehydrogenase e1 alpha subunit
Citric acid cycle
Oryza sativa (rice)
fructose-bisphosphate aldolase, class I
Defense against stress
Cucumis sativus (cucumber)
Oryza sativa Japonica Group
F1-ATP synthase, beta subunit
Oryza sativa Japonica Group
FS080420 library SmFL Solanum melongena cDNA clone
GSTSUB_UP_031_F12_01SEP2004_086 GSTSUB Artemisia annua cDNA, mRNA sequence
Homologs of unknown proteins
Accession No. a
NCBI accession No. b
FIP1 [V]-like protein
translational elongation factor EF-TuM
pentatricopeptide repeat-containing protein At1g02060
putative protein phosphatase 2 C
Oryza sativa Japonica Group
Structural proteins and enzymatic proteins involved in energy metabolism
Most of the identified proteins were structural proteins (non-enzymatic proteins) and enzymes involved in energy metabolism. These enzymes seem to have particularly important roles in cucumber plants under hypoxic conditions. The expressions of cytoplasmic aconitate hydratase (spot 4), pyruvate dehydrogenase 2 (spot 20), cytoplasmic malate dehydrogenase (spot 22) and pyruvate dehydrogenase e1 alpha subunit (spot 27) from the TCA cycle were downregulated under hypoxic conditions, but upregulated under hypoxia + CaCl2 conditions. The TCA cycle is a key component of the metabolic pathway by which all aerobic organisms generate energy by oxidization of pyruvate into carbon dioxide and water. Pyruvate dehydrogenase 2 (PDH2), a pyruvate dehydrogenase E1 beta isoform, and pyruvate dehydrogenase e1 alpha subunit are involved in the formation of cellular energy through the TCA cycle and in the synthesis of acetylcholine (acety1-CoA). Acetyl-CoA may then be used in the TCA cycle to carry out cellular respiration; thus, pyruvate dehydrogenase links the glycolytic pathway to the TCA cycle and releases energy via NADH. Calcium activates pyruvate dehydrogenase, isocitrate dehydrogenase (IDH) and α-ketoglutarate dehydrogenase . Aconitate hydratase (aconitase) catalyses the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the TCA cycle [26, 27]. Malate dehydrogenases (MDH), which is essential to the TCA cycle, catalyses the conversion of oxaloacetate to malate . Increased malate levels in plants contributes to plant acid resistance and aluminum toxicity tolerance . In apples and tomatoes, significant accumulation of malate dehydrogenases gene transcripts is related to plant and cell growth, as well as to tolerance of salt stress . The activities of enzymes such as succinate dehydrogenase (SDH), isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH) in the TCA cycle are different between the hypoxia and hypoxia + CaCl2 conditions, where exogenous calcium promotes the actives of SDH and IDH in cucumber . Moreover, calcium acts to maintain higher activities of MDH and SDH and a certain level of aerobic respiration in pepper . Thus, exogenous calcium seems to induce tolerance to hypoxia in cucumber plants through the activation of the enzymes involved in the TCA cycle.
Cytosolic enolase is expressed in many plant species in response to various environmental stresses, such as salt stress , cold [34, 35] and drought . However, the level of protein expression appears not to be correlated with the enzyme’s activity. Although enolase activity was increased by the stresses, the level of expression of the enolase protein showed no fluctuation  or was even observed to significantly decrease . In the present study, this enolase (spots 10 and 11) appeared to be downregulated under hypoxia compared to hypoxia + CaCl2 and control. These results imply that enolase activity is regulated at the posttranscriptional level under anaerobic conditions and its relative amount is increased by exogenous calcium during hypoxia.
The expression of phosphoglycerate mutase (PGAM) (spot 8) and cofactor-independent phosphoglyceromutase (iPGAM) (spot 16) markedly decreased under hypoxic stress, but increased on the addition of exogenous calcium. PGAM is a key enzyme in glycolysis, catalyzing the interconversion of the phosphate group from C-3 to C-2, which results in the conversion of 3-phosphoglycerate (3PGA) to 2-phosphoglycerate (2PGA). PGAMs are divided into two evolutionarily unrelated groups based on whether they require 2, 3-biphosphoglycerate as a cofactor: cofactor dependent PGAMs (dPGAMs) and cofactor-independent PGAM (iPGAMs). The iPGAMs are commonly present in higher plants, some invertebrates, fungi, and bacteria . PGAMs are important to stomatal movement, vegetative biomass production, and reproduction in Arabidopsis . Transgenic potato plants with reduced iPGAM enzyme activity showed reduced growth because of a reduced photosynthetic rate . These phenomena suggest that the conversion of 3PGA to 2PGA may be inhibited under hypoxia, and that exogenous calcium may increase the abundance of the proteins.
Fructose-bisphosphate aldolase (FBP aldolase, spot 30) is also an essential enzyme involved in glycolysis. It catalyzes a reversible cleavage reaction of fructose-1, 6-bisphosphate (F-1, 6-BP) into two trioses: glyceraldehydes-3-phosphate and dihydroxyacetone phosphate (DHAP) . Increased FBP aldolase activity stimulates the glycolytic pathway and plays an important role in gibberellin A (GA)-induced growth of rice roots  and in signal transduction . In the present study, downregulation of FBP aldolase under the hypoxia altered the levels of glycolysis and inhibited the growth of cucumber roots. Exogenous calcium significantly elevated the quantity of the FBP aldolase, which may help alleviate the effects of hypoxic stress. This result is consistent with the expression profile of this protein in cucumber roots under salt stress .
The glycolytic pathway is the major source of energy when oxygen availability decreases below the level at which oxygen becomes limiting for oxidative phosphorylation . Pyruvate produce from glycolysis is consumed by fermentative metabolism, which involves pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH, spot 26). ADH catalyzes the reduction of pyruvate to ethanol and results in continuous NAD+ regeneration. ADH is considered essential for survival of plants during anaerobic conditions . Ruthenium red, an organelle calcium channel blocker, dramatically reduced anoxia-induced ADH activity  and gene expression . As expected, the quantity of ADH was increased under hypoxia and increased further under hypoxia + CaCl2.
Spot 12, spot 18 and spot 36 were identified as starch synthase III (SSIII), galactokinase and fructokinase, respectively. SS is involved in the elongation of the linear chains of starch . SSIII specifically catalyzes the formation of chains with a degree of polymerization (DP) of 12 to 25. Other SS isoforms cannot fulfill this function . Galactokinase is involved in the conversion of stachyose to sucrose in the cucumber peduncle . Fructokinase specifically catalyzes the transfer of a phosphate group from ATP (the substrate) to fructose as the initial step in its utilization. Recent studies have suggested that sucrose and hexoses (mainly glucose and fructose) can act as sensing-molecules to elicit sugar responses in both source and sink organs when plants are under abiotic stress , and can control distinct aspects of plants’ development . In the present study, these enzymes were significantly downregulated under hypoxia, but upregulated under hypoxia + CaCl2. A decrease of starch or carbon metabolic activity was also observed in other studies using various plant species under hypoxia [44, 53]. These observations provide a convenient explanation of the adaptive response of plants to hypoxia, namely that plants limit their energy consumption by suppressing the synthesis of storage substances, such as starch and protein [54, 55]. Calcium seems to enhance carbohydrate metabolism and induces sugar signaling to enhance tolerance of cucumber plants subjected to hypoxic stress.
According to recent studies using transgenic plants, overexpression or altered expression of glutamine synthetase (GS) promotes the development of plants  such as wheat  and Lotus corniculatus . The expression of this protein (GS, spot 19) decreased under hypoxia, but was significantly enhanced under hypoxia + CaCl2. Thus, calcium appears to regulate nitrogen (N) metabolism through GS to relieve O2-deficient conditions in cucumber plants subjected to hypoxia.
ATP synthases (ATPases) are membrane-bound enzyme complexes/ion transporters that combine ATP synthesis and/or hydrolysis with the transport of protons through the membrane , playing a key role in biological energy metabolism. ATPases differ in respect to function (ATP synthesis and/or hydrolysis), structure (F-, V- and A-ATPases contain rotary motors) and in the type of ions they transport [59, 60]. Two ATPases, i.e. V-type proton ATPase catalytic subunit A (spot 5) and F1-ATP synthase, beta subunit (spot 33) were remarkably decreased under hypoxia. Under hypoxia + CaCl2, the level of the former (spot 5) was restored and the latter (spot 33) showed a tendency to be somewhat restored. These restored levels did not reach the level of the control (normoxic conditions). V-type proton ATPases generate a proton electrochemical gradient, which is the driving force utilized by the tonoplast Na+/H+ antiporter, to compartmentalize Na+ into the vacuole . F1-ATPases in mitochondria, chloroplasts and bacterial plasma membranes are the prime producers of ATP, using the proton gradient generated by oxidative phosphorylation (mitochondria) or photosynthesis (chloroplasts). Mitochondrial Ca2+ accumulation triggers activation of mitochondrial metabolism, which increases ATP synthesis in mitochondria and ATP levels in cytosol . This phenomenon suggests that hypoxia dramatically inhibits energy metabolism in cucumber plants, and in the case of these two ATPases, calcium cannot completely restore them to normoxic levels.
Calcium is an essential element for cell growth and plays a role as a second messenger in signal transduction pathways . Therefore, it is not surprising that calcium is implicated in plant metabolism regulation signaling, particularly in association with oxygen deprivation . According to Gao et al., exogenous calcium induces the promotion of physiologically active factors and matters in muskmelon plants, as compared to the factors and matters observed in plants under hypoxic stress after 6 days . CaCl2 pretreatment increased the accumulation of amino acids in rice roots under anaerobic stress, possibly via a Ca-Camodulin complex involved in the transduction of an anaerobic signal that inhibits proteolysis and solute release . In addition, downregulation of a suite of energy metabolic pathways, and therefore, oxygen-consumption, is a class of plant hypoxic responses . In the present study, enzymes of carbon and nitrogen metabolism in the cytosol, mitochondria and chloroplasts were significantly induced by exogenous calcium. Thus, calcium enhances the tolerance of cucumber plants under hypoxia by regulating metabolic systems in the glycolytic pathway and the TCA cycle, and the activity of enzymes, such as ADH and GS. Although exogenous calcium had only a slight effect on ATPases, this effect seems to be part of the global effect of calcium on metabolism in cucumber plants.
Plant growth and productivity is suppressed by hypoxic stress or flooding . As the cell metabolism adapts to hypoxia, increased protein degradation might control the levels of one or more regulators/enzymes . Protein phosphatase 2C (PP2Cs, spot 17) decreased under hypoxia and increased under hypoxia + CaCl2. This enzyme is a negative regulator of stress signaling in plants and mammals  and acts predominantly through the signaling pathway of the stress hormone, abscisic acid (ABA) . Thus, the increase in the amount of the enzyme under hypoxia + CaCl2 may imply that the exogenous calcium influences ABA signaling to relieve hypoxic stress. Spot 28 was identified as methionyl-tRNA synthetase (MetRS). This enzyme is a multi-domain protein that specifically binds tRNAMet and catalyzes the synthesis of methionyl-tRNAMet , giving it a vital role in protein biosynthesis. The MetRS gene has been described in the mitochondria and chloroplasts of Arabidopsis thaliana  and has been linked with plant cell anti-oxidant defense during oxidative stress . Although MetRS was not detected under normoxic conditions, it was induced under hypoxia and further increased under hypoxia + CaCl2. This result suggests that the expression of MerRS under hypoxia represents a stress response of the cucumber seeding and calcium enhanced this response under hypoxic stress.
Proteins related to the stress response
Excessive generation of reactive oxygen species (ROS) or oxidative stress is an integral part of many stress situations, including hypoxia . Higher plants have active oxygen-scavenging systems, consisting of multiple defense enzymes that can modulate the steady-state level of ROS . Peroxidase (POD, spot 31), a ubiquitous enzyme  present in plants, microbes, fungi and vertebrates. This enzyme acts as a biological catalyst to scavenge H2O2 . The activities and gene expressions of superoxide dismutase (SOD) and ascorbate peroxidase (APX) were increased in pigeon pea , mung bean  and cucumber [79, 80] under waterlogged conditions. These phenomena were consistent with the changes in the expression of POD in the present study. In addition, the expression of POD was further increased by treatment with calcium. Thus, exogenous calcium can enhance the activities of ROS-scavenging enzymes to defend against the damage caused by ROS, which would suppress the effects of hypoxic stress.
Validation of differentially expressed proteins by western blotting
Transcript accumulation patterns for 12 candidate proteins
Proteomic analysis is an effective means for clarifying protein expression patterns and permits the identification of candidate proteins. In the present study, calcium was demonstrated to be involved in the short-term hypoxic tolerance of cucumber plants. Exogenous calcium enhanced the expression of proteins involved in glycolysis, the TCA cycle, nitrogen metabolism, protein synthesis, fermentative metabolism and ROS defense. This phenomenon suggests that exogenous calcium could induce hypoxia tolerance by improving enzyme activity in systems related to respiratory metabolism and stress defense in cucumber plants. However, western blotting and RT-PCR analyses showed different results for the candidate proteins. In general, exogenous calcium improves the hypoxia tolerance of plants via multiple systems that are regulated by multiple genes relating to various metabolic and signaling pathways. The present study provides evidence of the mitigating effect of exogenous calcium on the growth and metabolic activities of cucumber plants restrained under hypoxia. Further proteomic studies in this area are clearly warranted and are ongoing.
Plant materials and growth conditions
Cucumber (Cucumis sativus L. cv. Jinchun No.2, hypoxia sensitive ) seeds were sterilized with 0.5% (W/V) sodium hypochlorite solution for 10 min and then washed thoroughly with deionized water. The washed seeds were sown on two layers of wet filter paper and incubated in the dark at 28°C for 24 h. The germinated seedlings were transplanted to plastic trays (41 × 41 × 5 cm) containing quartz sand and grown at 25–30°C (day) and 15–18°C (night), with 60–75% relative humidity (RH), in a greenhouse of Nanjing Agriculture University in 2010. The seedlings were supplied with 1/2-strength Hoagland’s nutrient solution (pH 6.5 ± 0.1, EC 2.0–2.2 dS m−1). At the 2nd leaf development stage, relatively uniform seedlings were transferred to tanks containing full strength Hoagland’s nutrient solution. The solution was renewed every 3 days. The solution in the tanks was kept at 20–25°C and aerated with an air pump to keep the dissolved oxygen (DO) level at 8.0 ± 0.2 mg L−1 (the optimum DO level for cucumber). At the 3rd leaf development stage, seedlings were subjected to one of three treatments. (1) Control: 1/2 Hoagland’s solution (containing 2 mM Ca2+) with DO of 8.0 ± 0.2 mg L−1. (2) Hypoxia treatment: 1/2 Hoagland’s solution (containing 2 mM Ca2+) with DO of 1.0 ± 0.1 mg L−1, which was prepared by pumping N2-gas into the nutrient solutions as the hypoxic treatment. The oxygen concentration in the nutrient solutions was monitored with an automatic DO control system (Quantum-25, Quantum Analytical Instruments Inc., USA). (3) Hypoxia + CaCl2 treatment: 1/2 Hoagland’s solution + 4 mM CaCl2 with DO of 1.0 ± 0.1 mg L−1. The oxygen concentration in the nutrient solutions was controlled as in the hypoxia treatment.
For analysis of total protein, root samples were harvested 3 days after the end of hypoxic treatment. Protein extraction was performed according to a modified version of the method of Hurkman . Root samples (1–2 g fresh weight) were ground in a mortar with liquid nitrogen. The ground samples were suspended in 30 mM 2-amino-2-(hydroxymethyl)-1,3-propanediole (Tris)-HCl (pH 8.7) containing 1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA), 1 mM dithiothreitol (DTT) and 1 mM phenylmethyl sulfonyl fluoride (PMSF), and then centrifuged at 15,000 g for 20 min. An aliquot (1 ml) of the resulting supernatant was placed into a tube and precipitated with acetone containing 10% TCA and 0.07% β-mercaptoethanol. The resulting protein sample was allowed to precipitate overnight at −20°C and then centrifuged at 20,000 g for 25 min. The pellet was rinsed three times with cold acetone containing 0.07% β-mercaptoethanol and allowed to stand at −20°C for 1 h. Finally, the protein pellet was air-dried and used for 2-DE.
Isoelectric focusing (IEF) was performed according to the methods of Duncan and Hershey  and O’Farrell . The dried protein pellet was rehydrated in rehydration buffer: 7 M urea, 2 M thiourea, 4% 3-[(3-cholanidopropyl) dimethylammonio]-1-propanesulfonic acid (CHAPS) (w/v), 40 mM DTT, 0.5% (v/v) immobilized pH gradient (IPG) buffer 4–7 and 0.01% (w/v) bromophenol blue. Protein levels were quantified according to the Bradford method . IPG strips of nonlinear pI 4–7 (13 cm) were loaded with 250 μl of protein sample containing 800 μg protein in a rehydration tray for 12–16 h at room temperature. Following rehydration, the IPG strips were run on an Ettan IPGphor 3 (GE Healthcare, USA). The voltage for IEF was set at 200 V for 1 h, followed by 500 V for 1 h, 1000 V for 1 h, 3000 V for 30 min, 5000 V for 30 min, gradient 8000 V for 30 min, and 8000 V rapid focus, reaching a total of 35,000 V h. The cell temperature was maintained at 20°C with a maximum current of 50 μA per strip. After running the first dimension, IEF strips were equilibrated for 15 min with 10 ml DTT buffer containing 6 M urea, 30% (v/v) glycerol, 2% SDS, 1% (w/v) DTT and 50 mM Tris–HCl (pH8.8) and then with iodoacetamide buffer with 2.5% (w/v) iodoacetamide instead of DTT for 15 min.
The second dimensional SDS-polyacrylamide gel electrophoresis (SDS–PAGE) was carried out on running gels (Hoefer SE600 Ruby Standard Vertical System, GE Healthcare; 12.5% polyacrylamide) in the presence of SDS, as described by Laemmli . The strips were embedded on the top of the SDS-gel and then sealed using a 1% molten agarose solution. Electrophoresis was carried out at 15 mA per gel until the bromophenol blue dye front reached about 1 cm from the bottom of the gel.
Image acquisition and analysis
For Coomassie brilliant blue (CBB) R-250 staining, the gels were fixed overnight in a mixture of MeOH–H2O (1:1, v/v) and AcOH:H2O (1:9, v/v) and then stained for 2 h in a mixture of AcOH:H2O(1:9, v/v) and 0.1% (w/v) CBB R-250. The stained gels were destained in a mixture of MeOH-H2O (1:1, v/v) and AcOH: H2O (1:9, v/v). The CBB-stained 2-D gels were scanned using an Image scanner III (GE Healthcare). The digitized images were analyzed with Imagemaster™ 2D Platinum version 5.0 (GE Healthcare). At least three gels from each treatment in three independent experiments were used for the analysis. The intensities of spots were quantified based on their relative volume, which was determined by the ratio of the volume of a single spot to the whole set of spots. Only spots with significant (at least 1.5-fold quantitative changes) and reproducible changes in three replicates were used for mass spectrometry. Student’s t-test and a significance level of 95% were used for the statistical analysis of the gels. Only the spots showing a statistically significant difference in protein abundance between the treatments were considered differentially expressed spots.
In-gel protein digestion, mass spectrometry and database search
Differentially expressed protein spots were excised from gels and transferred to sterilized 0.5 ml tubes. The excised protein spots were destained for 20 min with 100 mM NH4HCO3 in 30% acetonitrile (ACN) and then washed in Milli-Q H2O. The spots were kept in 0.2 M NH4HCO3 for 20 min and then lyophilized and rehydrated. Each spot was digested overnight in 30 μl of 50 mM NH4HCO3 containing 50 ng trypsin (Promega, Madison, WI, USA). After overnight digestion at 37°C, the peptides were extracted three times with a mixture of 50% ACN and 0.1% CF3CO2H (TFA). Extracts were pooled together and lyophilized. The resulting lyophilized tryptic peptides were kept at −80°C until mass spectrometric analysis.
MALDI-TOF/TOF MS analysis and database searching
MS and MS/MS spectra were obtained using the ABI 4800 Proteomics Analyzer MALDI-TOF/TOF (Applied Biosystems, Foster City, CA, USA) operating in a result-dependent acquisition mode. Peptide mass maps were acquired in positive ion reflector mode (20 kV accelerating voltage) with 1000 laser shots per spectrum. Monoisotopic peak masses were automatically determined within the mass range 800–4000 Da, with a signal to noise ratio minimum set to 10 and a local noise window width of m/z 250. The most intense ions were selected as precursors for MS/MS acquisition, excluding common trypsin autolysis peaks and matrix ion signals. In MS/MS-positive ion mode, spectra were averaged, collision energy was 2 kV, and default calibration was set. Monoisotopic peak masses were automatically determined with a signal to noise ratio minimum set to 5. The MS, together with MS/MS spectra were searched against the NCBI viridiplantae (V.2010.12.10, 184045 sequences) and NCBI EST viridiplantae databases (V.2010.12.10, 1847412 sequence) using the software GPS Explorer™, version 3.6 (Applied Biosystems) and MASCOT version 2.1 (Matrix Science, London, UK). The parameters used for searching were: trypsin cleavage, one missed cleavage allowed; carbamidomethyl (C) set as a fixed modification; oxidation of methionines allowed as variable modification; peptide mass tolerance within 100 ppm; fragment tolerance set to ± 0.3 Da; and minimum ion score confidence interval for MS/MS data set to 95%.
Total RNA was extracted from roots as described in the TRI reagent protocol (Takara Bio Inc). For all samples, total RNA (1 μg) was converted to cDNA using a Superscript first-strand synthesis system for RT-PCR according to the manufacturer’s instructions (Takara Bio Inc).
Primer sequences used in RT-PCR
Product length (bp)
Annealing temp. (°C)
Western blot analysis
The protein was extracted from roots using a mixture containing 0.5 M Tris–HCl (pH 6.8), 20% (v/v) glycerol, 2% (w/v) SDS, 5% (v/v) β-mercaptoethanol and 0.01% (w/v) bromophenol blue. The extracted protein was quantified by the Bradford method , denatured at 95°C for 3–5 min and then stored at −20°C until analysis.
SDS-PAGE was performed according to the method of Laemmli . After electrophoresis, protein bands were visualized with Coomassie blue R250. For western blot analysis, proteins (15 μg from each sample), separated by SDS-PAGE as above, were transferred to a 0.45 μm PVDF membrane and detected with antibodies (produced in rabbit; Univ-bio, Shanghai, China) raised against ADH (AS10_685), PDC (AS10_691), and SAM (positive control). The membrane was blocked with 5% nonfat dry milk for 2 h and washed with TBST three times. The membrane was then probed with the appropriate rabbit primary antibody at a 1: 2000 dilution in TBST supplemented with 5% nonfat dry milk. After an overnight incubation at 4°C, the membrane was washed with TBST and incubated at room temperature for 1 h with a Goat Anti-Rabbit IgG HRP-conjugate (1:1000 dilution with 5% dry milk) in TBST. The membrane was then washed with TBST three times and developed using diamino benzidene (DAB) and H2O2.
The research was support by the National Basic Research Program of China (973 Program) (No.2009CB119000), the National Nature Science Foundation of China (No.30871736, 30900995, 31071831, and 30571263), and partially supported by the earmarked foundation for Modern Agro-industry Technology Research System (No.CARS-25-C-03).
- Bailey-Serres J, Voesenek LACJ: Flooding stress: acclimations and genetic diversity. Annu Rev Plant Biol 2008, 59: 313–339. 10.1146/annurev.arplant.59.032607.092752View Article
- Sairam R, Kumutha D, Ezhilmathi K, Deshmukh P, Srivastava G: Physiology and biochemistry of waterlogging tolerance in plants. Biologia Plantarum 2008, 52: 401–412. 10.1007/s10535-008-0084-6View Article
- Kang YY, Guo SR, Duan JJ: Effects of root zone hypoxia on respiratory metabolism of cucumber seedlings roots. Chin J Ecol 2008, 19: 583–587.
- Pedersen O, Rich SM, Colmer TD: Surviving floods: leaf gas films improve O2 and CO2 exchange, root aeration, and growth of completely submerged rice. Plant J 2009, 58: 147–156. 10.1111/j.1365-313X.2008.03769.xView Article
- Mommer L, Visser EJW: Underwater photosynthesis in flooded terrestrial plants: a matter of leaf plasticity. Ann Bot 2005, 96: 581–589. 10.1093/aob/mci212View Article
- Kennedy RA, Rumpho ME, Fox TC: Anaerobic metabolism in plants. Plant Physiol 1992, 100: 1. 10.1104/pp.100.1.1View Article
- Li J, Sun J, Yang Y, Guo S, Glick BR: Identification of hypoxic-responsive proteins in cucumber roots using a proteomic approach. Plant Physiol Biochem 2012, 51: 74–80.View Article
- Henriksson E, Nordin Henriksson K: Salt-stress signalling and the role of calcium in the regulation of the Arabidopsis ATHB7 gene. Plant Cell Environ 2005, 28: 202–210. 10.1111/j.1365-3040.2004.01263.xView Article
- Aurisano N, Bertani A, Reggiani R: Involvement of calcium and calmodulin in protein and amino acid metabolism in rice roots under anoxia. Plant Cell Physiol 1995, 36: 1525–1529.
- Gao H, Chen G, Han L, Lin H: Calcium influence on chilling resistance of grafting eggplant seedlings. J Plant Nutr 2005, 27: 1327–1339. 10.1081/PLN-200025833View Article
- Yemelyanov V, Shishova M, Chirkova T, Lindberg S: Anoxia-induced elevation of cytosolic Ca2+concentration depends on different Ca2+sources in rice and wheat protoplasts. Planta 2011, 234: 271–280. 10.1007/s00425-011-1396-xView Article
- Subbaiah CC, Bush DS, Sachs MM: Mitochondrial contribution to the Anoxic Ca2+ signal in maize suspension-cultured cells. Plant Physiol 1998, 118: 759–771. 10.1104/pp.118.3.759View Article
- Gao H, Guo S: Influence of calcium on antioxidant system and nitrogen metabolism of muskmelon seedlings under nutrient solution hypoxia. Acta Hort. (ISHS) 2004, 691: 321–328.
- He LZ, Guo SR, Lu XM, Wang LP, Yang YJ: Effects of calcium on soluble protein expression of cucumber seedlings under roo-zoon hypoxia stress. J Nanjing Agric Univ 2012, 35: 21–25.
- Evans NH, McAinsh MR, Hetherington AM, Knight MR: ROS perception in Arabidopsis thaliana: the ozone-induced calcium response. Plant J 2005, 41: 615–626. 10.1111/j.1365-313X.2004.02325.xView Article
- Walz C, Giavalisco P, Schad M, Juenger M, Klose J, Kehr J: Proteomics of curcurbit phloem exudate reveals a network of defence proteins. Phytochemistry 2004, 65: 1795–1804. 10.1016/j.phytochem.2004.04.006View Article
- Buhtz A, Kolasa A, Arlt K, Walz C, Kehr J: Xylem sap protein composition is conserved among different plant species. Planta 2004, 219: 610–618.View Article
- Segarra G, Casanova E, Bellido D, Odena MA, Oliveira E, Trillas I: Proteome, salicylic acid, and jasmonic acid changes in cucumber plants inoculated with Trichoderma asperellum strain T34. Proteomics 2007, 7: 3943–3952. 10.1002/pmic.200700173View Article
- Donnini S, Prinsi B, Negri A, Vigani G, Espen L, Zocchi G: Proteomic characterization of iron deficiency responses in Cucumis sativus L. roots. BMC Plant Biol 2010, 10: 268. 10.1186/1471-2229-10-268View Article
- Du CX, Fan HF, Guo SR, Tezuka T, Li J: Proteomic analysis of cucumber seedling roots subjected to salt stress. Phytochemistry 2010, 71: 1450–1459. 10.1016/j.phytochem.2010.05.020View Article
- Todaka D, Kanekatsu M: Analytical method for detection of beta-amylase isozymes in dehydrated cucumber cotyledons by using two-dimensional polyacrylamide gel electrophoresis. Anal Biochem 2007, 365: 277. 10.1016/j.ab.2007.03.026View Article
- Juszczuk IM, Rychter AM: BN-PAGE analysis of the respiratory chain complexes in mitochondria of cucumber MSC16 mutant. Plant Physiol Biochem 2009, 47: 397–406. 10.1016/j.plaphy.2008.12.022View Article
- Li Q, Huang J, Liu S, Li J, Yang X, Liu Y, Liu Z: Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar. Proteome Sci 2011, 9: 44. 10.1186/1477-5956-9-44View Article
- Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C, Bergkamp R, Dirkse W, Van Staveren M, Stiekema W: Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature 1998, 391: 485. 10.1038/35140View Article
- Denton RM, Randle PJ, Bridges BJ, Cooper RH, Kerbey AL, Pask HT, Severson DL, Stansbie D, Whitehouse S: Regulation of mammalian pyruvate dehydrogenase. Mol Cell Biochem 1975, 9: 27–53. 10.1007/BF01731731View Article
- Beinert H, Kennedy M: Aconitase, a two-faced protein: enzyme and iron regulatory factor. FASEB J 1993, 7: 1442–1449.
- Flint DH, Allen RM: Iron − sulfur proteins with nonredox functions. Chem Rev 1996, 96: 2315–2334. 10.1021/cr950041rView Article
- Yao YX, Li M, Zhai H, You CX, Hao YJ: Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis. J Plant Physiol 2011, 168: 474–480. 10.1016/j.jplph.2010.08.008View Article
- Tesfaye M, Temple SJ, Allan DL, Vance CP, Samac DA: Overexpression of Malate Dehydrogenase in Transgenic Alfalfa enhances organic acid synthesis and confers tolerance to aluminum. Plant Physiol 2001, 127: 1836–1844. 10.1104/pp.010376View Article
- Yao YX, Dong QL, Zhai H, You CX, Hao YJ: The functions of an apple cytosolic malate dehydrogenase gene in growth and tolerance to cold and salt stresses. Plant Physiol Biochem 2011, 49: 257–264. 10.1016/j.plaphy.2010.12.009View Article
- Hu XH, Li J, Guo SR, Li J: Effects of Ca2+ on respiratory metabolism in roots of cucumber seedlings under root-zone hypoxia stress. Acta Hortic Sin 2007, 33: 1113–1116.
- Zhang ER, Ren YY, Hu HQ, Liu YH, Chen SS: Effects of calcium on growth and respiratory metabolism of hot pepper seedling roots under flood stress. Acta Hortic Sin 2009, 36: 1749–1754.
- Yan S, Tang Z, Su W, Sun W: Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics 2005, 5: 235–244. 10.1002/pmic.200400853View Article
- Lee DG, Ahsan N, Lee SH, Lee JJ, Bahk JD, Kang KY, Lee BH: Chilling stress-induced proteomic changes in rice roots. J Plant Physiol 2009, 166: 1–11. 10.1016/j.jplph.2008.02.001View Article
- Zhao J, Zuo K, Tang K: cDNA Cloning and Characterization of Enolase from Chinese Cabbage, Brassica campestris ssp. Pekinensis. Mitochondrial DNA 2004, 15: 51–57.
- Riccardi F, Gazeau P, de Vienne D, Zivy M: Protein changes in response to progressive water deficit in maize. Plant Physiol 1998, 117: 1253–1263. 10.1104/pp.117.4.1253View Article
- Forsthoefel NR, Cushman M, Cushman JC: Posttranscriptional and Posttranslational Control of Enolase Expression in the Facultative Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum L. Plant Physiol 1995, 108: 1185–1195. 10.1104/pp.108.3.1185View Article
- Jedrzejas MJ: Structure, function, and evolution of phosphoglycerate mutases: comparison with fructose-2,6-bisphosphatase, acid phosphatase, and alkaline phosphatase. Prog Biophys Mol Biol 2000, 73: 263–287. 10.1016/S0079-6107(00)00007-9View Article
- Zhao Z, Assmann SM: The glycolytic enzyme, phosphoglycerate mutase, has critical roles in stomatal movement, vegetative growth, and pollen production in Arabidopsis thaliana. J Exp Bot 2011, 62: 5179–5189. 10.1093/jxb/err223View Article
- Westram A, Lloyd JR, Roessner U, Riesmeier JW, Kossmann J: Increases of 3-phosphoglyceric acid in potato plants through antisense reduction of cytoplasmic phosphoglycerate mutase impairs photosynthesis and growth, but does not increase starch contents. Plant Cell Environ 2002, 25: 1133–1143. 10.1046/j.1365-3040.2002.00893.xView Article
- Matsumoto M, Ogawa K: New insight into the Calvin Cycle Regulation Glutathionylation of Fructose Bisphosphate Aldolase in Response to Illumination. In Photosynthesis Energy from the Sun. Edited by: Allen JF, Gantt E, Golbeck JH, Osmond B. Springer, Netherlands; 2008:872–874.
- Konishi H, Kitano H, Komatsu S: Identification of rice root proteins regulated by gibberellin using proteome analysis. Plant Cell Environ 2005, 28: 328–339. 10.1111/j.1365-3040.2005.01269.xView Article
- Schaeffer GW, Sharpe FT, Sicher RC: Fructose 1,6-bisphosphate aldolase activity in leaves of a rice mutant selected for enhanced lysine. Phytochemistry 1997, 46: 1335–1338. 10.1016/S0031-9422(97)00470-6View Article
- Rocha M, Licausi F, Araujo WL, Nunes-Nesi A, Sodek L, Fernie AR, van Dongen JT: Glycolysis and the Tricarboxylic Acid Cycle are linked by Alanine Aminotransferase during Hypoxia Induced by Waterlogging of Lotus japonicus. Plant Physiol 2010, 152: 1501–1513. 10.1104/pp.109.150045View Article
- Johnson JR, Cobb BG, Drew MC: Hypoxic Induction of Anoxia Tolerance in Roots of Adh1 Null Zea mays L. Plant Physiol 1994, 105: 61–67.View Article
- Subbaiah CC, Zhang J, Sachs MM: Involvement of Intracellular Calcium in Anaerobic Gene Expression and Survival of Maize Seedlings. Plant Physiol 1994, 105: 369–376. 10.1104/pp.105.1.369View Article
- Nie X, Durnin D, Igamberdiev A, Hill R: Cytosolic calcium is involved in the regulation of barley hemoglobin gene expression. Planta 2006, 223: 542–549. 10.1007/s00425-005-0094-yView Article
- Szydlowski N, Ragel P, Raynaud S, Lucas MM, Roldán I, Montero M, Muñoz FJ, Ovecka M, Bahaji A, Planchot V, et al.: Starch Granule Initiation in Arabidopsis Requires the Presence of Either Class IV or Class III Starch Synthases. The Plant Cell Online 2009, 21: 2443–2457. 10.1105/tpc.109.066522View Article
- Zhang X, Szydlowski N, Delvallé D, D’Hulst C, James M, Myers A: Overlapping functions of the starch synthases SSII and SSIII in amylopectin biosynthesis in Arabidopsis. BMC Plant Biol 2008, 8: 96. 10.1186/1471-2229-8-96View Article
- Miao M, Xu X, Chen X, Xue L, Cao B: Cucumber carbohydrate metabolism and translocation under chilling night temperature. J Plant Physiol 2007, 164: 621–628. 10.1016/j.jplph.2006.02.005View Article
- Rosa M, Prado C, Podazza G, Interdonato R, González JA, Hilal M, Prado FE: Soluble sugars—Metabolism, sensing and abiotic stress: A complex network in the life of plants. Plant Signal Behav 2009, 4: 388. 10.4161/psb.4.5.8294View Article
- Eveland AL, Jackson DP: Sugars, signalling, and plant development. J Exp Bot 2011,63(9):3367–3377.View Article
- Van Dongen JT, Roeb GW, Dautzenberg M, Froehlich A, Vigeolas H, Minchin PEH, Geigenberger P: Phloem Import and Storage Metabolism Are Highly Coordinated by the Low Oxygen Concentrations within Developing Wheat Seeds. Plant Physiol 2004, 135: 1809–1821. 10.1104/pp.104.040980View Article
- Gupta KJ, Zabalza A, Van Dongen JT: Regulation of respiration when the oxygen availability changes. Physiol Plant 2009, 137: 383–391. 10.1111/j.1399-3054.2009.01253.xView Article
- Geigenberger P: Response of plant metabolism to too little oxygen. Curr Opin Plant Biol 2003, 6: 247–256. 10.1016/S1369-5266(03)00038-4View Article
- Miflin BJ, Habash DZ: The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. J Exp Bot 2002, 53: 979–987. 10.1093/jexbot/53.370.979View Article
- Habash DZ, Massiah AJ, Rong HL, Wallsgrove RM, Leigh RA: The role of cytosolic glutamine synthetase in wheat. Ann Appl Biol 2001, 138: 83–89. 10.1111/j.1744-7348.2001.tb00087.xView Article
- Vincent R, Fraisier V, Chaillou S, Limami MA, Deleens E, Phillipson B, Douat C, Boutin JP, Hirel B: Overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic Lotus corniculatus L. plants triggers changes in ammonium assimilation and plant development. Planta 1997, 201: 424–433. 10.1007/s004250050085View Article
- Cross RL, Müller V: The evolution of A-, F-, and V-type ATP synthases and ATPases: reversals in function and changes in the H+/ATP coupling ratio. FEBS Lett 2004, 576: 1–4. 10.1016/j.febslet.2004.08.065View Article
- Rappas M, Niwa H, Zhang X: Mechanisms of ATPases-A multi-disciplinary approach. Curr Protein Pept Sci 2004, 5: 89–105. 10.2174/1389203043486874View Article
- Chinnusamy V, Jagendorf A, Zhu JK: Understanding and improving salt tolerance in plants. Crop Sci 2005, 45: 437–448. 10.2135/cropsci2005.0437View Article
- Jouaville LS, Pinton P, Bastianutto C, Rutter GA, Rizzuto R: Regulation of mitochondrial ATP synthesis by calcium: evidence for a long-term metabolic priming. Proc Natl Acad Sci 1999, 96: 13807–13812. 10.1073/pnas.96.24.13807View Article
- Chung HJ, Ferl RJ: Arabidopsis alcohol dehydrogenase expression in both shoots and roots Is conditioned by root growth environment. Plant Physiol 1999, 121: 429–436. 10.1104/pp.121.2.429View Article
- Rhoads DM: Plant mitochondrial retrograde regulation. In Plant Mitochondria. Volume 1. Edited by: Kempken F. Springer, New York; 2011:411–437. Advances in Plant BiologyView Article
- Gao H, Jia Y, Guo S, Lv G, Wang T, Juan L: Exogenous calcium affects nitrogen metabolism in root-zone hypoxia-stressed muskmelon roots and enhances short-term hypoxia tolerance. J Plant Physiol 2011, 168: 1217–1225. 10.1016/j.jplph.2011.01.022View Article
- Peter G: Response of plant metabolism to too little oxygen. Curr Opin Plant Biol 2003, 6: 247–256. 10.1016/S1369-5266(03)00038-4View Article
- Drew MC, He CJ, Morgan PW: Programmed cell death and aerenchyma formation in roots. Trends Plant Sci 2000, 5: 123–127. 10.1016/S1360-1385(00)01570-3View Article
- Smalle J, Kurepa J, Yang P, Babiychuk E, Kushnir S, Durski A, Vierstra RD: Cytokinin growth responses in Arabidopsis involve the 26S Proteasome subunit RPN12. The Plant Cell Online 2002, 14: 17–32. 10.1105/tpc.010381View Article
- Vlad F, Rubio S, Rodrigues A, Sirichandra C, Belin C, Robert N, Leung J, Rodriguez PL, Laurière C, Merlot S: Protein phosphatases 2C regulate the activation of the Snf1-related kinase OST1 by Abscisic acid in Arabidopsis. The Plant Cell Online 2009, 21: 3170–3184. 10.1105/tpc.109.069179View Article
- Lammers T, Lavi S: Role of Type 2C Protein Phosphatases in Growth Regulation and in Cellular Stress Signaling. Crit Rev Biochem Mol Biol 2007, 42: 437–461. 10.1080/10409230701693342View Article
- Havrylenko S, Legouis R, Negrutskii B, Mirande M: Methionyl-tRNA synthetase from Caenorhabditis elegans: A specific multidomain organization for convergent functional evolution. Protein Sci 2010, 19: 2475–2484. 10.1002/pro.529View Article
- Menand B, Maréchal-Drouard L, Sakamoto W, Dietrich A, Wintz H: A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl–tRNA synthetase in Arabidopsis thaliana. Proc Natl Acad Sci 1998, 95: 11014–11019. 10.1073/pnas.95.18.11014View Article
- Millar H, Considine MJ, Day DA, Whelan J: Unraveling the Role of Mitochondria During Oxidative Stress in Plants. IUBMB Life 2001, 51: 201–205. 10.1080/152165401753311735View Article
- Sairam RK, Dharmar K, Lekshmy S, Chinnusamy V: Expression of antioxidant defense genes in mung bean (Vigna radiata L.) roots under water-logging is associated with hypoxia tolerance. Acta Physiol Plant 2010, 33: 735–744.View Article
- Parida AK, Das AB: Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ Saf 2005, 60: 324–349. 10.1016/j.ecoenv.2004.06.010View Article
- Saraiva JA, Nunes CS, Coimbra MA: Purification and characterization of olive (Olea europaea L.) peroxidase – evidence for the occurrence of a pectin binding peroxidase. Food Chem 2007, 101: 1571–1579. 10.1016/j.foodchem.2006.04.012View Article
- Ikehata K, Buchanan ID, Pickard MA, Smith DW: Purification, characterization and evaluation of extracellular peroxidase from two Coprinus species for aqueous phenol treatment. Bioresour Technol 2005, 96: 1758–1770. 10.1016/j.biortech.2005.01.019View Article
- Sairam R, Kumutha D, Ezhilmathi K, Chinnusamy V, Meena R: Waterlogging induced oxidative stress and antioxidant enzyme activities in pigeon pea. Biol Plant 2009, 53: 493–504. 10.1007/s10535-009-0090-3View Article
- Kang Y, Guo S, Li J, Duan J: Effects of 24-Epibrassinolide on antioxidant system in cucumber seedling roots under hypoxia stress. Agric Sci China 2007, 6: 281–289. 10.1016/S1671-2927(07)60046-7View Article
- Wang CY, Guo SR, Liu CJ: Effects of calcium on expression of defense enzymes isoenzymes in roots of cucumber seedlings under root-zone hypoxic stress. Acta Bot Boreali-Occidentalia Sin 2009, 9: 1874–1880.
- Mustroph A, Boamfa E, Laarhoven L, Harren F, Albrecht G, Grimm B: Organ-specific analysis of the anaerobic primary metabolism in rice and wheat seedlings. I: dark ethanol production is dominated by the shoots. Planta 2006, 225: 103–114. 10.1007/s00425-006-0333-xView Article
- Kang YY, Guo SR, Li J, Duan JJ: Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia. Plant Growth Regul 2009, 57: 259–269. 10.1007/s10725-008-9344-xView Article
- Yan SP, Zhang QY, Tang ZC, Su WA, Sun WN: Comparative Proteomic Analysis Provides New Insights into Chilling Stress Responses in Rice. Mol Cell Proteomics 2006, 5: 484–496.View Article
- Ma YH, Guo SR: On different hypoxia tolerance of thirteen cucumber varieties. Jiangsu Agric Sci 2004, 5: 68–70.
- Hurkman WJ, Tanaka CK: Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol 1986, 81: 802. 10.1104/pp.81.3.802View Article
- Duncan R, Hershey JWB: Evaluation of isoelectric focusing running conditions during two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis: variation of gel patterns with changing conditions and optimized isoelectric focusing conditions. Anal Biochem 1984, 138: 144–155. 10.1016/0003-2697(84)90783-8View Article
- O’Farrell PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975, 250: 4007.
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72: 248–254. 10.1016/0003-2697(76)90527-3View Article
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227: 680–685. 10.1038/227680a0View Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.