Figure 1

The number of identified proteins in fractions of 1-D and 2-D pre-fractionation of glomerular proteins. The number of proteins identified in each of the 90 fractions separated by 1-D (SDS-PAGE) and 2-D pre-fractionation (solution phase isoelectric focusing followed by SDS-PAGE) are shown in 1-D and 2-D protein array format in which each cell or fraction is defined with Mw and pI range. The workflow for the protein identification strategy is depicted in Additional file 1, and details of identified proteins are shown in Additional file 4 and Additional file 5.