Figure 2
From: Profiling and annotation of human kidney glomerulus proteome
Distribution of calculated molecular weight of proteins identified in each fraction separated by SDS-PAGE in 2-D prefractionation of glomerular proteins. Fractions obtained by solution phase isoelectric focusing (fractions 1 to 5) were separated on a 10% SDS-PAGE gel (2-D prefractionation of glomerular proteins), and stained with Coomassie Brilliant blue. Each of the five lanes was cut into 15 slices, and in-gel digested with trypsin for LC-MS/MS analysis. Proteins identified in each of the 5 slices at the same position across the 5 lanes (S1 to S15) were integrated and used to construct a histogram based on calculated (theoretical) Mw as indicated. The red arrow indicates the observed molecular mass range.