Figure 3
From: Profiling and annotation of human kidney glomerulus proteome
Distribution of calculated isoelectric point of identified proteins among fractions of solution phase isoelectric focusing. Proteins fractionated by solution phase IEF (fractions 1 to 5) were separated on a 10% SDS-PAGE gel (2-D prefractionation of glomerular proteins), and stained with Coomassie Brilliant blue. Each of the five lanes was cut into 15 slices, and in-gel digested with trypsin for LC-MS/MS analysis. All the proteins identified in each of the 5 IEF fractions (F1 to F5) were integrated, and used to construct a histogram based on calculated (theoretical) pI as indicated. The red arrow indicates the observed pI range.