Figure 4
From: Profiling and annotation of human kidney glomerulus proteome
Location of identification of four key molecules related to the slit diaphragm in fractions of 2-D prefractionation of glomerular proteins. Distribution of nephrin, podocin, Neph1, and Fyn, known to be key components of the slit diaphragm of podocytes, is shown in the 2-D protein array. Sections, in which these proteins were identified, are highlighted with red colour gradation corresponding to the number of peptides matched to respective proteins. The calculated (theoretical) Mw and pI are indicated below the 2-D protein array for each of the four proteins.