Comparisons of protein profiles of beech bark disease resistant and susceptible American beech (Fagus grandifolia)

Mason, Mary E; Koch, Jennifer L; Krasowski, Marek; Loo, Judy

doi:10.1186/1477-5956-11-2

Proteome Science

Table 4 Protein spots analyzed by MS/MS

From: Comparisons of protein profiles of beech bark disease resistant and susceptible American beech (Fagus grandifolia)

Spot number	Tree & core number	Protein ID or EST homology [PRIDE accession: 17706] ^a	NCBI or EST Accession Number ^b	Mascot Score ^c	Percent Coverage	Matching peptides	Peptide sequence ^d	Peptide score ^e	m/z	Mr(expt)
109*^f	1504_B6	polyphenol oxidase	ABWP2_088861_1753_2628	180	33	2	TEFAGSFVNVPSK	72.2	692.1743	1382.334
						4	GVATIGGIK	47.2	408.4638	814.913
						2	GVATIGGIK IQFVS	233.8	695.8282	1389.6418
						2	IQFVS	26.9	593.343	592.3357
307	1504_H5	unnamed protein product	gi\|157327346	735	44	2	NILFVISK PDVFK	71.3	760.6617	1519.3088
						4	SPTSDTYVIFGEAK	73.9	758.1431	1514.2716
						6	IEDLSSQLQTQAAEQFK	107.3	969.241	1936.4674
						2	DIELVM TQAGVSR	75.7	718.1302	1434.2458
						4	DIELVMTQAGVSR	98.1	709.7012	1417.3878
307	1504_H5	alpha nascent polypeptide	CCWP2_066819_3631_1428	255	52	2	NILFVISK PDVFK	71.3	760.6617	1519.3088
		associated complex				4	SPTSDTYVIFGEAK	74.8	758.1431	1514.2716
						5	IEDLSSQLQTQAAEQFK	108.5	969.241	1936.4674
505	1504_A6	none	none
1004*	CM02d_C1	thioredoxin	WOA_067025_0312_1563	120	25	2	VNTDESSSIATR	76.5	640.5275	1279.0404
						2	STLTTSIEK	46.2	490.4947	978.9748
2101*	1504_G7	ribonuclease activity regulator	CCNHS_110619_3244_2001	214	24	2	VFEDNVLVR	57.1	546.048	1090.0814
						2	VLVVDGGGSLR	67.6	537.0695	1072.1244
2101*	1504_G7	dimethylmenaquinone	gi\|15232963	199	21	4	ALQPVFQIYGR	46.8	647.1753	1292.336
		methyltransferase family protein				2	VLVVDGGGSLR	78.9	537.0695	1072.1244
						2	DVDEINGCDIGVR	66.5	731.6575	1461.3004
2101*	CM02d_C3	Eukaryotic translation initiation	gi\|3024017	75	19	1	ELVFK EDGQEYAQVLR	45.4	642.4005	1924.1797
		factor 1A				2	DYQDDK ADVILK	52.4	712.098	1422.1814
3101	CM02d_E5	heat shock protein	ROA_067935_0424_3607	120	22	2	ADLPGLK	39.2	357.4087	712.8028
		[homologous match]				1	K EEVK VEVEEGR	32.3	477.7563	1430.2471
						2	EEVK VEVEEGR	53.2	652.0981	1302.1816
4103	1504_F5	17.7 kDa heat shock protein	gi\|1235898	238	26	2	ADLPGLK	39.7	357.3622	712.7098
						7	K EEVK VEVEEGR	79.9	715.8541	1429.6936
						5	EEVK VEVEEGR	58.7	652.0201	1302.0256
						5	VLQISGER	30.3	451.5257	901.0368
						5	AAMENGVLTVTVPK	32.4	723.6349	1445.2552
5001	CM02d_F5	stress and pathogenesis-related	gi\|3901018	858	65	3	GVFTYESENTSVIPPAR	84.8	934.2761	1866.5376
		protein				13	AFVLDADNLIPK	30.5	659.2544	1316.4942
						2	AFVLDADNLIPK VAPQSIK	42.0	680.6581	2038.9525
						7	STETLEGDGGPGTIK	44.6	731.3245	1460.6344
						5	STETLEGDGGPGTIK K	66.3	795.6741	1589.3336
						3	K ITFGEGSQFK	60.5	414.6845	1241.0317
						4	ITFGEGSQFK	49.7	1113.4546	1112.4473
						1	ISYEIK IVASPDGGSLLK	25.6	630.7996	1889.377
						6	IVASPDGGSLLK	63.3	578.6285	1155.2424
						2	GNHEIK EEEVK	55.8	656.6091	1311.2036
						10	AVEAYLLAHPDAYN	47.7	774.1621	1546.3096
5002	1504_G5	superoxide dismutase	gi\|38228697	263	40	1	HAGDLGNVNVGDDGTVSFTIIDK	72.8	782.4584	2344.3534
						4	QIPLCGPNSIIGR	53.1	713.6349	1425.2552
						4	AVVVHGDPDDLGK	55.5	661.611	1321.2074
						2	STGNAGGRIACGIIGLQG	45.4	851.7599	1701.5052
						4	IACGIIGLQG	45.6	1001.4746	1000.4673
5303*	1504_F7	triosephosphate isomerase	ABWP2_034459_1554_1107	250	52	3	VASPAQAQEVHFGLR	66.9	805.7179	1609.4212
						3	K WLQVNTSPEVAATTR	48.9	601.354	1801.0402
						1	IIYGGSVNGANCK	63.6	677.621	1353.2274
7107*	1504_B7	unknown, CBS domain protein	CCWP1_110480_2642_0865	274	53	2	VGDIM TEENK	49.3	576.5307	1151.0468
						2	VGDIM TEENK LITVTLDTK	59.3	713.168	2136.4822
						1	LITVTLDTK	45.3	502.5542	1003.0938
						2	GM IGM VSIGDVVR	84.6	683.4301	1364.8456
						2	LNAFIQGGY	38.5	982.3546	981.3473
7110*	1504_C7	unknown, CBS domain protein	CCWP1_110480_2642_0865	230	53	2	VGDIM TEENK	56.2	576.5154	1151.0162
						2	VGDIM TEENK LITVTLDTK	61.3	713.1682	2136.4828
						2	GM IGM VSIGDVVR	80.2	683.6461	1365.2776
						2	LNAFIQGGY	40.0	982.4321	981.4248
7203	CM02d_H3	none
7408*	CM02d_A4	annexin	ACHS1n_159819_2640_1758	209	66	2	WTSSNQVLMEIACTR	84.0	906.6581	1811.3016
						2	SSHDLLLAR	37.8	506.6234	1011.2322
						1	SLEEDVAYHTTGDFR	28.8	580.6663	1738.9771
						2	YEGDEVNMTLAK	60.4	693.4765	1384.9384
7408*	CM02d_A4	isoflavone reductase like	ABWP1_026351_0296_1851	203	37	1	SKILIIGGTGYIGK	57.7	710.562	1419.1094
						2	ILIIGGTGYIGK	70.4	602.8907	1203.7668
						2	SGHPTFALVR	36.0	542.7687	1083.5228
						2	ESTVSDPVK	38.7	481.347	960.6794
7602*	CM02d_B4	dehydrin	ABWP1_045441_0451_3051	225	53	1	VFEKIPGAGNK	46.8	580.3038	1158.593
		[homologous match]				1	IPGAGNKDR	40.9	465.1174	928.2202
						2	DR VQGEQYR	41.0	576.0114	1150.0082
						1	GAM DKVFEK	30.4	521.1149	1040.2152
						2	IPGAGNKDK	43.2	450.2458	898.477
						2	DKVQGDQYR	34.7	555.0321	1108.0496
8002	CM02d_E4	none	none
8104*	1504_C9	glycine-rich RNA binding protein	ABWP1_015278_0178_1196	100	24	2	AFSPYGEILESK	49.6	671.1248	1340.235
		[homologous match]				2	NITVNEAQSR	50.5	566.5034	1130.9922
8104*	CM02d_F4	glycine-rich RNA-binding protein	ABWP1_015278_0178_1196	97	25	2	AFSPYGEILESK	50.4	671.1497	1340.2848
		[homologous match]				2	GFGFVTFSNEK	46.6	617.2156	1232.4166
8202	1504_D9	none	none
8303	1504_H8	none	none
8309	1504_A9	none	none
8309	CM02d_A2	none	none
8506*	1504_F8	glyceraldehyde 3-phosphate	ROB_045015_1217_3381	176	57	2	AASFNIIPSSTGAAK	39.8	718.1123	1434.21
		dehydrogenase, cytosolic				2	VPTVDVSVVDLTVR	82.6	750.6729	1499.3312
						2	FGIVEGLM TTVHSITATQK	54.0	684.0022	2048.9848
8607	1504_E8	glyceraldehyde-3-phosphate	gi\|120666	237	21	2	FGIVEGLMTTVHSITATQK	38.7	684.0699	2049.1879
		dehydrogenase, cytosolic				2	AASFNIIPSSTGAAK	47.5	718.1649	1434.3152
						2	VPTVDVSVVDLTVR	88.8	750.368	1498.7214
						4	AGIALNDNFVK	37.9	581.4758	1160.937
						2	LVSWYDNEWGYSTR	72.0	888.5875	1775.1604
8607	CM02d_C2	Ran binding protein	ABWP1_041027_0417_3079	146	33	4	LEEVAVTTGEEDETSILDLK	117.4	1096.213	2190.4114
						2	FDKDGNQWK	28.9	569.5167	1137.0188
9302*	1504_G8	germin-like/unknown/predicted	CCNHS_162322_3610_0519	282	48	3	IPGLNTLGVSLSR	85.5	663.9008	1325.787
						3	IDYAPGGLNPPHTHPR	77.0	872.1409	1742.2672
						2	SIKKGEIFVFPK	66.0	697.1452	1392.2758
						4	KGEIFVFPK	53.6	532.7322	1063.4498
9401	CM02d_B5	none

^aIdentification of the NCBI protein match, or for EST peptide matches, the BLASTP identification of the EST. Mass spectrometry data and identifications have been submitted to the PRIDE database. See the field ‘gel spot identifier’ in the Identification Detail View to review individual spot spectra and identifications.
^bNCBI number of the matching protein, or the Fagaceae genomics EST ID for peptided matching EST's.
^cMASCOT score is a probability based score. Reported scores are sums of peptide identifications that are significant (except homologous where noted), and higher scores are equivalent to lower probability of an equal or better match occurring at random.
^dUnderline indicates an oxidized residue. Bold indicates a missed trypsin cleavage.
^ePeptide mascot score for each peptide (or the highest match for multiple peptides).
^f * indicates spot with significant BBD effect and non-significant STAND and STANDxBBD interaction effect, and are the primary targets for biomarker development.

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ISSN: 1477-5956

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