Figure 5

Western blot validation. NME2, ATIC and RAB5 were analyzed with Western Blot to validate the MS results. The WB quantification displayed good correlation with MS results. Average NME2 induced/control ratio in MS was 0.55 and with WB 0.69. For ATIC, the ratios were 0.62 (MS) and 0.68 (WB) and for RAB5 the ratios were 0.66 (MS) and 0.69 (WB). Beta-actin was used as loading control. The Western Blot results were normalized to average control values. Bars represent the average of three biological repeats and the error bars represent lowest and highest WB quantification results of the three biological replicates.