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Table 1 Annotation and proposed metabolic pathways of the differential protein spots obtained from the MS-searching

From: Identification of differentially expressed proteins of Arthrospira (Spirulina) plantensis-YZ under salt-stress conditions by proteomics and qRT-PCR analysis

Spot

Spot

ORF

Accession no.

Gene product (best hit in NCBInr database)

MW (Da) (T/E)

PI (T/E)

Matched peptides

Cov

MASCT Score

E-value

Fold change

General function

CK701

M718

3277

ZP_03275759

FAD-dependent pyridine nucleotide disulphide oxidoreductase [Arthrospira maxima CS-328]

47147/42726

6.67/6.49

6

20%

78

0.018

−4.70

Posttranslational modification, protein turnover, chaperones

CK275

M305

2281

ZP_06380867

Peptidyl-prolyl cis-trans isomerase, cyclophilintype[Arthrospira str. Paraca]

23921/28931

4.74/4.49

6

36%

75

0.036

−3.65

M908

H653

2287

ZP_03274434

Molecular chaperone DnaK [Arthrospira maxima CS-328]

62452/57079

4.70/5.57

27

38%

150

1.00E-09

3.65

Fructose and mannose metabolism

CK461

H-N

4535

ZP_06381408

GDP-L-fucose synthase [Arthrospira str. Paraca]

35652/35258

5.59/6.14

23

53%

260

1.00E-20

/

Fatty acid biosynthesis

CK361

H-N

5055

ZP_06383266

Enoyl-(acyl carrier protein) reductase [Arthrospira str. Paraca]

27751/31814

5.57/5.83

11

37%

79

0.013

/

M344

H224

1433

YP_002521548

3-oxoacyl-[acyl-carrier protein] reductase [Thermomicrobium roseum DSM 5159]

28631/31269

6.54/5.34

9

34%

73

0.049

3.24

Photosynthesis - antenna proteins

CK47

H-N

4103

ZP_03271327

Phycobilisome core component [Arthrospira maxima CS-328]

17433/14807

6.26/5.74

14

84%

165

3.30E-11

/

CK342

M394

2155

ZP_06382427

Phycobilisome linker polypeptide [Arthrospir a str. Paraca]

29450/31199

9.25/5.68

15

55%

218

1.6E-16

−3.26

CK423

H-N

4633

ABV01983

Cpch [Arthrospira Sp-16]

30852/33929

7.82/6.29

25

60%

295

3.30E-24

/

CK467

H342

4632

ABB84420

Cpci [Arthrospira Sp-5]

32790/35621

8.33/4.59

19

53%

150

1.00E-09

3.09

CK-N

H69

4634

ZP_06380686

Phycocyanin, alpha subunit [Arthrospira maxima CS-328]

17703/19259

5.82/5.89

10

58%

91

0.00076

/

CK-N

M252

4635

ABD64607

Phycocyanin beta chain [Arthrospira]

18506/28381

5.19/4.58

11

55%

98

0.00016

/

Purine metabolism

CK-N

M137

1911

ZP_06383116

Adenylylsulfate kinase [Arthrospira str. Paraca]

19897/22320

5.22/5.46

16

79%

200

1e-14

/

Lysine biosynthesis

CK-N

M463

3910

ZP_06382240

Dihydrodipicolinate synthase [Arthrospira str. Paraca]

30869/34837

5.20/5.80

22

51%

141

8.3e-09

/

CK500

M548

4030

ZP_03276569

Diaminopimelate epimerase [Arthrospira maxima CS-328]

32452/37662

9.22/6.83

39

76%

310

1e-25

3.56

Glutathione metabolism

CK517

H384

4938

ZP_06381009

Glutathione synthetase [Arthrospira str. Paraca]

36159/38491

5.93/6.64

15

43%

100

0.0001

4.04

Glycolysis/Gluconeogenesis

CK-N

M595

3807

ZP_03274253

Glyceraldehyde-3-phosphate dehydrogenase, type I [Arthrospira maxima CS-328]

36534/39635

6.07/5.26

24

61%

110

0.00001

/

Cysteine and methionine metabolism

M778

H545

1688

ZP_06381331

Adenosylhomocysteinase [Arthrospira str. Paraca]

46667/45550

5.64/5.98

25

52%

245

3.3e-19

3.26

Oxidative phosphorylation

CK145

M140

2740

ZP_06381970

F-type H+-transporting ATPase subunit delta [Arthrospira str. Paraca]

20108/22132

6.15/6.79

15

53%

116

2.6e-06

3.77

CK109

M93

2739

ZP_03274295

F-type H+-transporting ATPase subunit β[Arthrospira maxima CS-328]

19538/17970

5.14/5.04

13

59%

114

4.2e-06

3.28

ABC transporters

M800

H564

2050

ZP_03275033

ABC-type nitrate/nitrite transport system substrate-binding protein [Arthrospira maxima CS-328]

48859/47107

4.68/4.46

26

50%

235

3.30E-18

3.57

Protein export

M-N

H185

4213

YP_798213

Signal recognition particle subunit SRP54[Leptospira borgpetersenii serovarHardjobovis L550]

49178/28565

7.08/5.46

8

24%

74

0.046

/

Transcriptional regulator

M111

H-N

1989

 

Transcriptional regulator, abrb family [Arthrospira maxima CS-328]

15532/19479

8.49/4.22

11

44%

90

0.0011

/

Function unknown

CK934

M-N

1251

ZP_06381540

Hypothetical protein aplap_07632 [Arthrospira str. Paraca]

67407/65336

5.66/6.20

19

42%

166

2.6E-11

/

CK424

M-N

5516

ZP_03272395

Conserved hypothetical protein [Arthrospira maxima CS-328]

41116/34016

6.04/6.08

13

50%

205

3.3E-15

/

CK283

M-N

3023

ZP_06383801

Hypothetical protein AplaP_19221 [Arthrospira platensis str. Paraca]

23973/29221

7.03/6.22

9

58%

94

0.00044

/

CK202

H127

1198

ZP_06382670

Hypothetical protein aplap_13413 [Arthrospira str. Paraca]

29241/25864

8.15/5.52

11

54%

103

5.2e-05

4.70

CK-N

M175

273

ZP_06384609

Hypothetical protein aplap_23383 [Arthrospira str. Paraca]

20229/25353

5.93/5.71

9

49%

121

8.3e-07

/

M839

H591

624

ZP_06384828

Hypothetical protein aplap_24525 [Arthrospira str. Paraca]

37661/50381

4.91/5.25

27

61%

226

2.60E-17

3.99

  1. Note: (1) 0.02 M NaCl for control group (CK); 0.5 M NaCl for medium salt treatment (M); and 1.0 M NaCl for high salt treatment (H).
  2. (2) CK-N, M-N and H-N represent no protein-spots are observed in control, medium and high salt treatment groups, respectively.
  3. (3) The grey mark indicates up-regulation of proteins.
  4. (4) The negative values in fold column indicate the down-regulation.
  5. (5) T/E indicates theoretical values/experimental values.