Figure 1

Four representative 2-D DIGE gel images. Exudate proteins were separated by 2-D DIGE using immobilised pH 4–7 gradients (24 cm, linear) in the first dimension and 12% SDS-PAGE in the second dimension. Figure 1 a shows all 136 significantly modulated spots across the three days post mortem (days 1, 3 and 7); the gel image is from an internal standard that consisted of a CyDye3-labelled mixture of the pooled sample. Figure 1 b, c and d show representative images from day 1, day 3 and day 7 post mortem respectively; all labelled with CyDye 5. Figure 1 b highlights 21 spots, of the 136 significantly changing, that have highest abundance at day 1, whereas Figure 1 c and d highlight respectively 3 spots that have a highest abundance at day 3 and 16 spots that have highest abundance at day 7 post mortem.