Table 4 Summary of PhosSA site assignment results using Mascot for AQP2 mass spectrometry data set using CID and HCD fragmentations (see text for description). N/A means that the AQP2 results were not found in the data set
From: PhosSA: Fast and accurate phosphorylation site assignment algorithm for mass spectrometry data
CID data sets | PhosSA Accuracy(%) | No. of peptides similar to Sequest | No. of peptides different than Sequest |
|---|---|---|---|
AQP2-H-(S256/S261) | 94.6 | 690 | 17 |
AQP2-M-(S256/S261) | 94.4 | 714 | 26 |
AQP2-L-(S256/S261) | 100 | 665 | 26 |
AQP2-H-(S264) | 100 | 658 | 9 |
AQP2-M-(S264) | 89 | 661 | 24 |
AQP2-L-(S264) | 60 | 801 | 17 |
HCD data sets | Â | ||
AQP2-H-(S256/S261) | 94.5 | 857 | 13 |
AQP2-M-(S256/S261) | 95.9 | 921 | 25 |
AQP2-L-(S256/S261) | 100 | 992 | 27 |
AQP2-H-(S264) | 100 | 822 | 14 |
AQP2-M-(S264) | 96.6 | 804 | 20 |
AQP2-L-(S264) | 75 | 935 | 12 |