Figure 1
From: PPIRank - an advanced method for ranking protein-protein interations in TAP/MS data
Overview of the tandem affinity purification coupled with mass-spectrometry (TAP/MS) technology. (A) A tagged protein is pulled down via its tag, together with associated proteins (red) and other non-specific interacting proteins (black). (B) The protein samples collected are then broken down into peptides by proteases and analyzed by mass-spectrometry. (C) A list of peptide sequences and corresponding proteins from each sample are reported as the results.