Skip to main content
Figure 2 | Proteome Science

Figure 2

From: SILAC labeling coupled to shotgun proteomics analysis of membrane proteins of liver stem/hepatocyte allows to candidate the inhibition of TGF-beta pathway as causal to differentiation

Figure 2

Validation of the expression of selected cell surface proteins in RLSCs and DH. (A) Western blot analysis in total cell extract (WCE), soluble (S) and membrane (M) fractions for the indicated proteins. For each fraction, 10 μg of protein sample were loaded; GAPDH and Calnexin expressions were used respectively as total and membrane proteins loading control. One representative experiment out of three is shown. (B) Bands were analyzed by densitometry using Quality-One software (Bio-Rad laboratories, Richmond, CA). The Y axis shows the relative intensity respect to GAPDH (WCE) and calnexin (M). All data were from at least three independent experiments and shown as mean ± SD.

Back to article page

Proteome Science

ISSN: 1477-5956