Figure 3
Linearity and accuracy of the self-build quantitative method for glycoprotein standards. The glycoprotein standards Fetuin and invertase were serially diluted to determine the linearity and accuracy of the self-build quantitative method. (A) The ratio of (Group A + Group B)/Group C. Glycopeptide concentration ratios calculated by the self-build method were significantly associated with the theoretical values, with both correlation coefficients (R2) being >0.99. (B) The ratio of Group A/Group C was calculated without considering back-labeling and incomplete labeling in glycoprotein standards; a difference was found between the result of the relative quantitation ratio of 18O/16O in (B) and the theoretical ratio.