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Table 6 Array 2: Averaged LLQs/ULQs in pg/mL obtained from 15 point standard titrations

From: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

Feature

B

W

A

W+A

LLQ (pg/mL)

ULQ (pg/mL)

AR

73

27

0

27

48

5599

BDNF

8

87

5

92

26

3956

Flt3Lig

2

98

0

98

17

9835

GCP-2

26

74

0

74

132

13153

HCC4

8

46

46

92

112

7907

I-309

30

70

0

70

20

5142

IL-17

95

5

0

5

330

7921

IL-1a

69

31

0

31

10

2943

IL-1b

46

54

0

54

4

2932

IL-2

89

7

4

11

31

3671

M-CSF

55

45

0

45

86

6761

MCP-1

17

69

14

83

82

2065

MIG

8

87

5

92

13

5030

MIP-1b

29

56

16

71

16

2399

MIP-1d

10

72

18

90

192

6969

NT-3

75

25

0

25

160

22314

NT-4

60

40

0

40

128

19170

PARC

4

25

70

96

12

1870

Rantes

0

13

87

100

5

1302

SCF

29

71

0

71

69

20306

TARC

15

85

0

85

21

3134

TNF-R1

2

97

1

98

108

16285

TNF-a

71

29

0

29

56

6901

TNF-b

88

12

1

12

221

7617

VEGF

86

14

0

14

702

79564

sgp130

0

63

37

100

334

38573

  1. Data shown is from titrations run in 8 client projects (1000 clinical samples surveyed) covering 8 diverse disease areas. The percentage of samples falling below (B), within (W) or above (A) the linear range of detection are presented. The analyte was considered to be detectable if the W+A percentage was above 50%.