Figure 2
From: Target selection of soluble protein complexes for structural proteomics studies
Denaturation-renaturation binding assay between NAC and APAF-1, TUCAN and CASPASE-9 detected by PAGE. Continuous PAGE analyses of CARD-containing proteins. An equal amount of proteins were incubated in a dialysis bag and dialyzed sequentially in a denaturation buffer, renaturation buffer and binding buffer (see material and methods). The protein complex formation was visualized by non-denaturating PAGE.