Figure 5

2-D electrophoresis determined expression changes of some enzymes involved in glycolysis (PFK, FBA, TPI, GAP, PGK, PGM, ENO and PYK) and TCA cycle (CitZ, CitB, CitC, OdhB, SucC, SucD, SdhA, FumA and MDH). The x-axis gives the time after xylose-induction and the y-axis gives the average value of normalized protein spot volume on 2-D gel. Abbreviations of protein names are referred to Table 1. Samples were taken from (A): a batch culture of B. megaterium MS941dsrS shortly before xylose-induction, then 1 h, 6 h and 10 h after induction for recombinant DsrS production (solid curves), and (B): a control batch culture of B. megaterium MS941dsrS without xylose-induction with the same time course of sampling as (A) (dashed curves).