Table 6 Kinetic parameters obtained by simulating the conditions for the Peptidomatrix, using equations A2 and A7 (two binding sites). Data in Fig 6.

Antibody 0.1

Antibody 1

Antibody 10

Antibody 100

Kd

25.53

25.22

22.69

14.70

Amax

0.52

4.80

27.49

61.77

B: increasing added antibody at four fixed levels of attached peptide

Attached peptide 0.1

Attached peptide 1

Attached peptide 10

Attached peptide 100

Kd

70.55

65.62

43.28

25.68

Amax

1.58

12.28

41.07

69.42

C: Competition assay – Kd determined when increasing added free peptide at four fixed levels of attached peptide

Attached peptide 0.1

Attached peptide 1

Attached peptide 10

Attached peptide 100

Kd

1.3869

1.4333

1.6399

1.8080

Amax

0.2291

1.9375

9.7190

23.5973

D: Competition assay – Kd determined when increasing free peptide at four fixed levels of added antibody

Antibody 0.1

Antibody 1

Antibody 10

Antibody 100

Kd

1.001

1.01

1.64

4.12

Amax

0.16

1.52

9.72

28.98