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Figure 3 | Proteome Science

Figure 3

From: An ELISA-based procedure for assaying proteins in digests of human leukocytes and cell lines, using specifically selected peptides and appropriate antibodies

Figure 3

Detection of the MDR1 peptide P494. In panel A calibration curves are plotted as optical density (OD450) vs. peptide concentration. The curve is the calculated regression fit, based on the hyperbolic equation detailed in the Materials and Methods section. The OD values of 50 μl of cell digest (approximately 4.5 million cells) from wild type and drug-resistant CEM cells are shown as solid and empty triangles respectively. For the sake of clarity we have added arrows to indicate the position of these points on the curve. In panels B the amounts of peptide (in nanograms) for a range of digest volumes (from 6.25 μl to 50 μl) are shown. These values have been calculated using the regression curve of panels A.

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ISSN: 1477-5956