Figure 2

Proteins in fractions enriched for plasma membrane vesicles or secretory vesicles from resting PMN. Secretory vesicles (a) and Plasma membrane vesicles (b) recovered by free-flow electrophoresis of isolated γ fraction from resting PMN were separated by PAGE on 4–20% SDS gradient gel and visualized by SYPRO ruby staining. The bands (indicated by numbers) from top to bottom in each lane were excised from the gel with the help of 1.5 mm band picker and processed with an automatic in-gel digester robot, ProGest as described in Materials and Methods. The numbers assigned to the bands in the gel correspond to the proteins listed in Table A1 (Additional file 2).