Figure 3
From: Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation
Representative 2D gel regions (A, B), 3D visualization of the spot contour in purple (C, D) and western blots (E) for NSF (left set of images) and alpha-SNAP (right set of images). Average ratio from 2D-DIGE analysis of the spot containing NSF was -1.33 (n = 9); average band volume ratio from western blot analysis was -1.28 (n = 3). Average ratio from 2D-DIGE analysis of the spot containing alpha-SNAP was -1.37 (n = 9); average band volume ratio from western blot analysis was -1.15 (n = 3). For all western blots, the variations in sample loading revealed by the actin signal is taken into account for the calculation of the average band volume ratio.