Table 1 Variation of Pooled Sample intensities from Individual Sample intensities, using independent clustering (A) or imposed clustering (B).
From: Pooling serum samples may lead to loss of potential biomarkers in SELDI-ToF MS proteomic profiling
Set, Significance level | Number of peak clusters in pooled samples retained for analysis | Median number of SDs pooled control cluster intensities are from mean individual sample intensities (IQR) | Median number of SDs pooled case cluster intensities are from mean individual sample intensities (IQR) | Median number of SEs pooled sample case to control ratios are from means of individual sample ratios (IQR) | Proportion of pooled sample case to control intensity ratios that lie less than 0.25 SEs from means of individual sample ratios |
|---|---|---|---|---|---|
A, all | 97/197 (40.9%) | 0.37 (0.18 – 0.61) | 0.39 (0.16 – 0.62) | 0.20 (0.10 – 0.33) | 59/97 (61%) |
A, p < 0.05 | 21/35 (60%) | 0.29 (0.12 – 0.37) | 0.33 (0.17 – 0.44) | 0.16 (0.11 – 0.36) | 13/22(62%) |
A, p < 0.05 (manually added peaks, see text) | 35/35 (100%) | 0.34 (0.16 – 0.56) | 0.33(0.18 – 0.57) | 0.20 (0.12 – 0.45) | 19/35 (54%) |
B, all | - | 0.40 (0.20 – 0.67) | 0.43 (0.19 – 0.80) | 0.21 (0.11 – 0.43) | 109/197 (55%) |
B, p < 0.05 | - | 0.30 (0.19 – 0.46) | 0.40 (0.19 – 0.58) | 0.14 (0.09 – 0.40) | 23/35 (66%) |