Multidimensional protein fractionation using ProteomeLab PF 2D™ for profiling amyotrophic lateral sclerosis immunity: A preliminary report

Table 2 Summary of results from mass spectrometry analyses.

Protein No	Protein	Fraction ID: Patient Sample	MW	NCBI Accession #	pI
1	Hemopexin	F1: 1A,2A,3B,3A F8: 1B,1A,2B,2A,3B F6: 1B,1A,2B,2A,3B,3A F5: 1B,1A,2B,2A,3B,3A F7: 1B,1A,2B,3B	51676	11321561	6.55
2	Plasminogen	F1: 1A,2A,3B,3A F4: 1A,2A,3B,3A F6: 2^a	90569	4505881	7.04
3	Anti-thrombin	F1: 2A,3B	13788	23978644	5.91
4	Histidine-rich glycoprotein precursor	F1: 1B,1A,3B F4: 1B,1A,2B,2A,3B,3A F9: 2B	59578	4504489	7.09
5	Complement Factor I	F5: 1A,2B,2°	65720	119392081	7.72
6	Complex of the Catalytic Domain of Human Plasmin and Streptokinase	F1: 1A,2A,3B F4: 1A,2B,2A,3B,3A F9: 2B	27286	5821850	8.27
7	Prealbumin	F1: 2A F5: 1B,2B,2A,3B	15919	219978	5.52
8	A Chain A, prealbumin	F1: 2A F8: 1B F5: 1B,2B,2A,3B	13760	230651	5.55
9	Beta-2 glycoprotein I apolipoprotein H	F1: 1A,3B F4: 1A,2A F6: 1A,2B,2A,3B	38312	28810	8.34
10	Gelsolin isoform a precursor	F1: 3B F9: 2B F6: 2B F5: 1B,3B,3A	85697	4504165	5.90
11	Kringle 2 Domain of Human Plasminogen	F4: 1A,2A,3B	9637	6573460	7.55
12	Complement factor H-related protein 1 precursor (FHR-1)	F6: 1A,2B,2A,3B F7: 2A	37661	543981	7.75
13	H factor (complement)-like 3	F6: 2A	30651	5031695	6.00
14	Retinol-binding protein 4, plasma precursor	F1: 2B F5: 2B	23010	55743122	5.76
15	Coagulation factor XII-Mie	F5: 2A	67735	24899162	8.03
16	Glutathione peroxidase 3 precursor	F5: 2A	25505	121672	8.20
17	Factor H	F7: 2A	139125	31965	6.28

Identification by LCMS/MS of proteins in plasma from three ALS patients before (B) and after (A) GA immunization. Only proteins with two or greater sequenced peptides are listed. Coordinates for Fraction IDs are listed in Table 1.

ISSN: 1477-5956