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Figure 3 | Proteome Science

Figure 3

From: Glycoproteomic analysis of two mouse mammary cell lines during transforming growth factor (TGF)-β induced epithelial to mesenchymal transition

Figure 3

Schematic of the lectin affinity workflow. Immobilized wheat germ agglutinin was used to enrich glycosylated proteins from BRI-JM01 and NMuMg cells with or without the induction of EMT by TGF-β. Peptides in the WGA eluant were then quantified from nano-LC-MS data using a software package developed in-house, MatchRx, to identify differentially-expressed peptides.

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Proteome Science

ISSN: 1477-5956