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Figure 4 | Proteome Science

Figure 4

From: Glycoproteomic analysis of two mouse mammary cell lines during transforming growth factor (TGF)-β induced epithelial to mesenchymal transition

Figure 4

Effective enrichment of glycoproteins by WGA lectin affinity workflow. Immobilized wheat germ agglutinin was used to enrich glycosylated proteins from BRI-JM01 and NMuMG cells with or without the induction of EMT by TGF-β. Proteins from control and TGF-β treated BRI-JM01 (A) and NMuMG (B) cells were fractionated on a WGA affinity column. To determine the efficiency of glycoprotein enrichment by WGA, proteins from the unfractionated cellular lysate (input, 'IN'), the WGA flow-through ('FT') fraction, and WGA elution ('EL') fraction were separated by SDS-PAGE and visualized by both a glycoprotein-specific stain and Sypro Ruby, a total protein stain.

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Proteome Science

ISSN: 1477-5956