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Figure 6 | Proteome Science

Figure 6

From: Glycoproteomic analysis of two mouse mammary cell lines during transforming growth factor (TGF)-β induced epithelial to mesenchymal transition

Figure 6

Representative LC-MS quantification data. Three-dimensional extracted ion chromatograms, produced by MSight [30] are shown for two peptides that are down-regulated upon the induction of EMT by TGF-β. (A) In BRI-JM01 cells, a doubly-charged peptide from the low-density lipoprotein receptor was identified with m/z of 970.0 m/z, representing the peptide sequence NIYWTDSVPGSVSVADTK. LC-MS data from the first 2 biological repetitions show consistently lower MS signal for this peptide upon TGF-β treatment. However, the peptide was not found in the third biological repeat. (B) In NMuMG cells, a doubly-charged peptide from alpha-N-acetylglucosaminidase was identified with m/z 1167.1, representing the sequence ALADESGLDTYSLSGGGGVPVLVR. The MS signal corresponding to this peptide is consistently lower after induction of EMT by TGF-β than in control cells.

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Proteome Science

ISSN: 1477-5956