Figure 2From: Proteins altered by elevated levels of palmitate or glucose implicated in impaired glucose-stimulated insulin secretion INS-1E cell SELDI-TOF mass spectra and 2D-PAGE map. SELDI-TOF mass spectrum (A) and 2D-PAGE map (B) generated from INS-1E cells cultured at 20 mM glucose in the absence or presence of 0.5 mM palmitate for 48 hours. Representative mass spectrum with magnified area of calmodulin with the tentative masses of 16.8, 17.0 and 17.2 kDa, (n = 6) from cells cultured at 20 mM glucose alone (black traces) or presence of palmitate (red traces). Identified proteins are marked in the gel image by their gene names. Mr, m/z and pI denote relative molecular mass, mass over charge and isoelectric point of the protein.Back to article page