Proteins altered by elevated levels of palmitate or glucose implicated in impaired glucose-stimulated insulin secretion

Table 2 Glucose and palmitate-regulated INS-1E proteins

Gene name	Protein name	Synonyms	M_r (Da)	pI
Calm1, Calm3	Calmodulin		16696	4,09
G6pdx, G6pd	Glucose-6-phosphate 1-dehydrogenase	G6PD EC 1.1.1.49	59794	5,97
Ganab	Alpha glucosidase 2 alpha neutral subunit		90857	5,77
Gars	Gars protein		72669	5,76
Hnrnpa3, Hnrpa3	Heterogeneous nuclear ribonucleoprotein A3	hnRNP A3	37291	8,46
Lonp1, Lon, Prss15	Lon peptidase 1	EC 3.4.21.-, Lon protease-like protein LONP, Mitochondrial ATP-dependent protease Lon, Serine protease 15	106296	6,17
Ndufv1	NADH dehydrogenase (ubiquinone) flavoprotein 1		51383	8,37
Pgk1, Pgk-1	Phosphoglycerate kinase 1	EC 2.7.2.3	44909	8,02
Psmc5	Proteasome p45/SUG		42174	7,11
Rab2	RAB2, member RAS oncogene family		18513	6,43
RGD1561681_predict.	Pyruvate kinase 3		58312	7,15
Tcp1, Cct1, Ccta	t-complex protein 1	CCT-alpha, TCP-1-alpha	60835	5,86

INS-1E cells were cultured at 5.5 or 20 mM glucose in the absence or presence of palmitate for 48 hours. After culture, cells were lysed and protein profiled. Differentially expressed proteins by glucose or palmitate were identified by peptide mass fingerprinting. M_r and pI denote relative molecular mass and isoelectric point of the protein.

ISSN: 1477-5956