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Table 1 Quantification of phosphorylations on TBP interactors in cell cycle-blocked cells.

From: Quantitative mass spectrometry of TATA binding protein-containing complexes and subunit phosphorylations during the cell cycle

Protein

Peptide

Position

Phospopeptide ratio (G2/M:AS)

Protein ratio (G2/M:AS)

Phosphopeptide/Protein ratio (G2/M:AS)

Reference

TAF1

TpGMDSNWWVLK

T876

150.91

1.40

107.99

this study

TAF3

RPLDSpPEAEELPAMK

S183

1.38

1.60

0.86

this study; ref [31]

TAF4

SpPGVQPQLVLGGAA-

QTASLGTATAVQTGTPQR

S543

3.13

1.42

2.20

this study

TAF7

YIESpPDVEKEVK

S159

15.77

1.36

11.58

this study

TAFI41/TAF1D

LAGDSpFIVSSEFPVR

S234

> 200

2.88

> 200

this study

Protein

Peptide

Position

Phospopeptide ratio (G1/S:AS)

Protein ratio (G1/S:AS)

Phosphopeptide/Protein ratio (G1/S:AS)

Reference

TAF3

RPLDSpPEAEELPAMK

S183

0.11

0.12

0.94

this study; ref [31]

TAF3

QIQTpPPDAGK

T364

0.05

0.12

0.39

this study

TAF3

RISpGPECTTPK

S427

0.02

0.12

0.19

this study

TAF6

AAAPPQPSpPPPTK

S532

0.07

0.11

0.62

this study

TAF6

QEAGDSpPPPAPGTPK

S653

0.12

0.11

1.13

this study; ref [32]

TAF7

YIESpPDVEKEVK

S159

0.11

0.17

0.63

this study

TAFI41/TAF1D

SRGSGFPFLESpENEK

S137

1.43

1.35

1.06

this study

TAFI41/TAF1D

TQCIPYSpPKGEK

S40

1.94

1.35

1.44

this study

  1. Phosphorylations (indicated with a 'p' behind the modified residue) detected on TBP interactors were quantified in cell cycle-blocked cells relative to asynchronous cells and were corrected for protein levels. The sequence of the tryptic peptide detected is indicated.