Proteome analysis of human Wharton's jelly cells during in vitro expansion

Table 1 Proteins no longer expressed after the 2^nd culture passage.

Spot #	Abbr. Name	AC^a	Protein Name	Score^b	SC^c %	pI	Mr(Da) Theo.	pI	Mr(Da) Exp.
2	PAI2	P05120	Plasminogen activator inhibitor 2	123	46	5,46	46596	5,44	30700
22	SHOT1	A0MZ66	Shootin 1	56	39	5,27	72109	4,80	50629
34	EIF3I	Q13347	Eukaryotic translation initiation factor 3 subunit I	214	61	5,38	36502	5,21	34955
35	ACTG	P63261	Gamma Actin	68	32	5,31	41792	4,98	32910
36	ACTB	P60709	Beta Actin	96	58	5,29	41736	5,04	33210
61	KAD5	Q9Y6K8	Adenylate kinase isoenzyme 5	81	37	5,23	22420	4,80	28831
67	VIME	P08670	Vimentin	100	35	5,06	53651	4,95	47492
75	KAD5	Q9Y6K8	Adenylate kinase isoenzyme 5	79	54	5,23	22420	4,70	27829

A) Entry code number from SWISS PROT database.
B) Score is -10*Log(P), where P is the probability that the observed match is a random event, it is based on Swiss-Prot database using the MASCOT searching program as MALDI-TOF data.
C)Sequence coverage means the ratio of portion sequence covered by matched peptide to the full length of the protein sequence.

ISSN: 1477-5956