Figure 6

Comparison of the HDL protein pattern from one subject before (t = 0) and after (t = 8) LPS exposure. HDL was isolated from plasma by density-gradient ultracentrifugation. Proteins (300 μg) were separated with 2-DE and detected by Sypro Ruby staining. The proteins were quantified as fluorescence intensity per total protein fluorescence. The four SAA isoforms, as identified by mass spectrometry (see Figure 7) shown in the t = 8 h image represent: (A) SAA-1α-desArg, -desArgSer and -desArgSerPhePhe; (B) SAA-1α; (C) SAA-2α-desArg, -desArgSer and -desArgSerPhePhe; (D) SAA-2α.