Figure 1

Schematic representation of peptidome enrichment and analysis. The methodology used for the enrichment and analysis of plasma peptides is shown. After enrichment of hydrophobic peptides using RPC-18 magnetic beads, the peptide fraction was subjected to mass spectrometry using two different acquisition modes. ClinPro Tools was used for model generation using pattern analysis of the peptide mass fingerprints of patients or controls and the differentiating peaks were identified using MS/MS. Following the discovery phase, different blinded samples were used for validation.