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Figure 3 | Proteome Science

Figure 3

From: Ligand-dependent differences in estrogen receptor beta-interacting proteins identified in lung adenocarcinoma cells corresponds to estrogenic responses

Figure 3

Validation of ERβ-interacting proteins by coimmunoprecipitation and western blot. A: H1793 and A549 cells were treated with 10 nM E2 for 1 h, whole cell extracts (WCE) were prepared and equal amts (30 μg protein) western blotted for EGFR. Higher EGFR protein expression was detected in A549 than H1793 cells. The arrow indicates the 170 kDa wild type EGFR. The membranes were stripped and reprobed for α-tubulin for normalization. The graph shows the quantification of EGFR expression. B: H1793 and A549 cells were treated with 10 nM E2, 10 ng/ml EGF, or the combination for 1 h. WCE of H1793 and A549 were incubated with rhERβ and then immunoprecipitated using a FLAG affinity beads or mouse IgG control and immunobloted using EGFR, calmodulin, and ERβ antibodies. EGFR bands were identified in the IP of H1793 cells treated with EtOH and E2, but not EGF or the combination of E2 and EGF treated.

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Proteome Science

ISSN: 1477-5956