Figure 4
From: Differential chromatin proteomics of the MMS-induced DNA damage response in yeast
Spotting growth assay for genotoxic sensitivity. The yeast cells were either knockout (ycp4, lsp1, pst2, bmh1, hsp31, acf2, ste4, and rnr4) or DAmP strains (rpa1, rpa2, yrb1 and arp3). The assay was performed on YPD plates containing indicated concentrations of MMS or HU. Cells were 10-fold serially diluted and incubated at 30°C for 2 days. An isogenic wild-type strain BY4741 was used as a negative control and rad53-11 mutant strain as a positive control.