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Table 1 Proteins identified within the chromatin enriched fraction.

From: Differential chromatin proteomics of the MMS-induced DNA damage response in yeast

Protein name

Enrichment factor

Estimated copies/cell a

Cellular Localization b

Description c

Arp3

+1.74

6650

Cytoskeleton, Nucleus [54]

Actin-related protein 3,

actin filament organization

Arp4

+4.20

1070

Nucleus

Actin-related protein 4,

chromatin remodeling

Arp7

+2.53

1360

Nucleus

Actin-related protein 7,

chromatin remodeling

Arp9

+3.35

1790

Nucleusd

Actin-related protein 9,

chromatin remodeling

Asc1

+2.88

333000

Cytoplasm

G protein beta subunit,

Small subunit ribosomal protein

Atp2

+1.43

164000

Mitochondrion

F1-ATPase beta chain, mitochondrial ATP synthesis

Cdc10

+3.09

14100

Septin ring, cytosketelon, nucleusd

Septin ring protein, cell division

Cgi121

+3.37

N.D.

Nucleus [27]

Component of KEOPS, telomere uncapping and elongation

Crn1

+4.06

2900

Contractile ring, Cytoskeletond

Coronin,

actin filament organization

Cys3

+1.65

38300

Cytoplasm

Gamma-cystathionase, Cysteine biosynthesis

Egd2

+1.55

38000

Cytoplasm, Nucleus [55]

Component of NAC, ribosome associated

End3

+10.4

2600

Cytoskeleton

EH domain protein, actin cytoskeletal organization

Gdi1

+1.58

7280

Cytoplasm

GDP dissociation inhibitor, vesicle mediated transport

Ilv2

+13.4

31900

Mitochondrion

Acetolactate synthase, amino acid synthesis

Ilv5

+1.95

883000

Nucleus, Mitochondriond

Acetohydroxy-acid isomerase, amino acid synthesis

Lat1

+2.42

5440

Mitochondrion

Dihydrolipoamide acetyl-transferase, pyruvate metabolism

Lsp1

+5.54

104000

Cytoplasm (punctate composite)

Component of eisosome, endocytosis

Pdb1

+3.93

9970

Mitochrondrion, Nucleoidd

Pyruvate dehydrogenase, pyruvate metabolism

Pil1

+1.65, Pil1(a) +2.31, Pil1(b)

115000

Cytoplasm (punctate composite)

Component of eisosome, endocytosis

Pst2

+3.37

2330

Mitochondrion,

Nucleus [56]

Flavodoxin-like protein

Qcr2

+1.73

35700

Mitochondrion

Ubiquinol cytochrome C reductase, respiration

Raf1

+3.37

N.D.

Nucleus [57]

FLP1recombinase activating factor, plasmid maintenance

Rpb3

+3.38

10000

Nucleus

DNA directed RNA polymerase II

Rpc40

+3.98

13000

Nucleus

Component of RNA polymerases I

Rpt1

+1.92

105

Nucleus

ATPase subunit of proteosome

Rvb2

+4.03

3030

Nucleusd

Transcription, chromatin remodeling

Stm1

+2.21

46800

Cytoplasm, Nucleusd

TOR signaling, telomere structure

Swi3

+6.85

3150

Nucleus

Chromatin remodeling complex, SWI/SNF

Taf14

+4.32

3120

Nucleus

Subunit of TFIID, TFIIF, INO80, SWI/SNF, NUA3 complexes, chromatin remodeling

Tfc7

+3.89

2660

Cytoplasm, Nucleus

RNA polymerase IIIc

Tub2

+3.73

N.D.

Nucleus, Cytoskeletond

Tublin 2, microtubule component

Ume1

+2.22

3040

Cytoplasm, Nucleus

Negative regulator of meosis, binding to histone deacetylase RPD3.

Ura7

+3.73

57600

Cytoplasm

CTP synthase, phospholipid biosynthesis

  1. DIGE was used to compare the chromatin fraction vs. whole cell yeast extract, and protein spots with an enrichment factor greater than +1.40 were selected for identification.
  2. a - Protein copy numbers per cell are from Ghaemmaghami et al. (2003) [3], obtained via the Saccharomyces Genome Database http://www.yeastgenome.org.
  3. b - unless otherwise noted, localizations were obtained from Huh et al (2003) [58] via the Saccharomyces Genome Database.
  4. c- functional descriptions were obtained from the Saccharomyces Genome Database.
  5. d- localization was obtained from the organelle database http://organelledb.lsi.umich.edu.