Figure 6

Determinations of macrophage cell area and F-actin measurements after SP-A treatment. SP-A KO alveolar macrophages were isolated, cultured overnight, and treated with SP-A (or its control). The cells were then fixed, permeabilized, stained with Alexa Fluor 488 phalloidin, and analyzed as described. A) Cell area is indicated for control and SP-A-treated macrophages after 6 hr of SP-A treatment. The data shown represent measurements of cell area made on cells (n = 115 cells for control; n = 102 cells for SP-A-treated) from 5 mice. T-test indicates a significant difference (*) between groups (p = 0.0016). B) F-actin levels are represented by mean fluorescence intensity/pixel. The data were obtained from analysis of the same number of mice and cells indicated in Panel A. The two groups were significantly different (*; p = 0.016). C) Cell area was determined from macrophages after one hour of SP-A treatment. Control values (n = 60 cells) and SP-A treated values (n = 62 cells) were significantly different (*; p < 0.0001). D) F-actin levels in the samples described in Panel C, as represented by mean fluorescence intensity/pixel, were significantly different (*; p < 0.0001).