In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement

Phelps, David S; Umstead, Todd M; Quintero, Omar A; Yengo, Christopher M; Floros, Joanna

doi:10.1186/1477-5956-9-67

Proteome Science

Table 2 Changes in actin-related/cytoskeletal (ARC) proteins (as compared to KO baseline).

From: In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement

Gel No.	Protein Name	KO 6 hr SP-A	KO 18 hr SP-A	WT
1	65-kDa macrophage protein	↓	↓*	↓
2	Actin related protein 2/3 complex, subunit 5	↓	UN	↑
3	Actin-related protein 3	↓*	↓*	↓
4	Actr2 protein	↓	↓	↓
6	Annexin A2	↓*	↓*	↓*
11	Calpain, small subunit 1	↓	↓*	↓
12	Capping protein (actin filament) muscle Z-line, alpha 2 (CapZ alpha-2)	↓	↑	↑
13	Capping protein (actin filament) muscle Z-line, beta isoform a (CapZ beta)	↓*	↓	↓
15	Chaperonin subunit 2 (beta)	↓	↓*	↓*
19	Chloride intracellular channel 1	↓	↓*	↓*
20	Chloride intracellular channel 4 (mitochondrial)	↑	↑*	↑
22	Coactosin-like 1	↑	↑*	↑
24	Eno1 protein (Alpha-enolase)	↑	↓	↓
25	Eukaryotic translation initiation factor 5A	↑	↑	↑
26	Ezrin	↓	↓*	↓*
27	F-actin capping protein alpha-1 subunit (CapZ alpha-1)	↑	↑	↑
30	Gamma-actin	↓	↓*	↓*
31	Gelsolin precursor	↑	↓	↑
33	Guanine deaminase	↓	↓	↓
34	Heat shock protein 1, beta	↑	↓	↑
39	Hematopoietic cell specific Lyn substrate 1	UN	↑	↑
45	Keratin complex 2, basic, gene 8	↓	↑	↑*
46	Keratin type II	↓	↓*	↓
47	Krt13 protein	↑	↑*	↓
49	Major vault protein (MVP)	↓*	↓*	↓*
50	Microtubule-associated protein, RP/EB family, member 1	↓	↓*	↓
51	Myosin light chain, regulatory B-like	↑	↑	↑
53	p50b; Leukocyte-specific protein 1 (LSP1)	↓	↓	↓
62	Put. beta-actin (aa 27-375)	↑	↑*	↑
63	Rab GDP dissociation inhibitor beta	↓	↓*	↓*
64	Rho GDP dissociation inhibitor (GDI) alpha	↑	↓	↓
65	Rho, GDP dissociation inhibitor (GDI) beta	↑	↑	↑
67	Stathmin	↑	↑	↑*
69	Tropomodulin 3	↑	↑	↑
70	Tropomyosin 3, gamma	↓	↓	↑
71	Tubulin, beta 5	↑	↓	↑
75	Valosin-containing protein	↓	↓*	↓*
76	Vimentin	↑	↓	↓*
	Total changes	21↓, 16↑, 1UN	24↓, 13↑, 1UN	21↓, 17↑
	Total significant changes	4↓*	13↓, 4↑	9↓, 2↑

Comparison of mean normalized volumes (see Additional File 4) for proteins from KO mice to KO 6 hr SP-A, KO 18 hr SP-A, and wild-type mice. Increased (↑), decreased (↓), unchanged (UN), determined to be significant (p < 0.05) by t-test (*).

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ISSN: 1477-5956

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