In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement

Table 4 Changes in protease balance/chaperone function (PBCF) proteins (as compared to KO baseline).

Gel No.	Protein Name	KO 6 hr SP-A	KO 18 hr SP-A	WT
11	Calpain, small subunit 1	↓	↓*	↓
14	Cathepsin D precursor	↓	↓	↓
15	Chaperonin subunit 2 (beta)	↓	↓*	↓*
19	Chloride intracellular channel 1	↓	↓*	↓*
21	CNDP dipeptidase 2	↓*	↓*	↓
22	Coactosin-like 1	↑	↑*	↑
24	Eno1 protein (Alpha-enolase)	↑	↓	↓
34	Heat shock protein 1, beta (HSP90AB1)	↑	↓	↓
35	Heat shock protein 5 precursor (GRP78)	↑	↓*	↓
36	Heat shock protein 65 (HSP60)	↓	↓*	↓*
37	Heat shock protein 8 (HSC70, HSC71)	↑	↑	↓
38	Heat shock protein 90, beta (Grp94), member 1	↓	↓*	↓*
55	Prolyl 4-hydroxylase, beta polypeptide precursor	↑*	↑	↓*
56	Proteasome (prosome, macropain) 28 subunit, alpha	↓	↑	↑
57	Proteasome alpha 1 subunit	↓	↓	↓
58	Protein disulfide isomerase associated 6	↓	↓	↓
59	Protein disulfide-isomerase A3 precursor	↓	↓	↓
66	Serine (or cysteine) proteinase inhibitor, clade B, member 1a	↓	↑	↑
75	Valosin-containing protein	↓	↓*	↓*
	Total changes	13↓, 6↑	14↓, 5↑	16↓, 3↑
	Total significant changes	2↓*	8↓, 1↑	6↓*

Comparison of mean normalized volumes (see Additional File 4) for proteins from KO mice to KO 6 hr SP-A, KO 18 hr SP-A, and wild-type mice. Increased (↑), decreased (↓), unchanged (UN), determined to be significant (p < 0.05) by t-test (*).

ISSN: 1477-5956