In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement

Table 5 Changes in regulatory/differentiative processes (RDP) proteins (as compared to KO baseline).

Gel No.	Protein Name	KO 6 hr SP-A	KO 18 hr SP-A	WT
25	Eukaryotic translation initiation factor 5A	↑	↑	↑
41	Heterogeneous nuclear ribonucleoprotein K	↓*	↓*	↓*
42	High mobility group 1 protein	↑	↓	↑
43	Hnrpf protein	↓	↓	↓
44	Kappa-B motif-binding phosphoprotein	↓	↓	↓
52	Nucleophosmin 1	↓	↓	↓
53	p50b	↓	↓	↓
60	Protein synthesis initiation factor 4A	↑	↓	↓*
	Total changes	5↓, 3↑	7↓, 1↑	6↓, 2↑
	Total significant changes	1↓*	1↓*	2↓*

Comparison of mean normalized volumes (see Additional File 4) for proteins from KO mice to KO 6hr SP-A, KO 18hr SP-A, and wild-type mice. Increased (↑), decreased (↓), unchanged (UN), determined to be significant (p < 0.05) by t-test (*).

ISSN: 1477-5956