In vivo rescue of alveolar macrophages from SP-A knockout mice with exogenous SP-A nearly restores a wild type intracellular proteome; actin involvement

Table 6 Changes in Nrf2-regulated (NRF) proteins (as compared to KO baseline).

Gel No.	Protein Name	KO 6 hr SP-A	KO 18 hr SP-A	WT
1	65-kDa macrophage protein	↓	↓*	↓
14	Cathepsin D precursor	↓	↓	↓
20	Chloride intracellular channel 4 (mitochondrial)	↑	↑*	↑
28	Ferritin heavy chain 1	↓	↑*	↓
29	Ferritin light chain 1	↓*	↓	↓
30	Gamma-actin	↓	↓*	↓*
31	Gelsolin precursor	↑	↓	↑
32	Glucose-6-phosphate dehydrogenase X-linked	↓	↓*	↓*
34	Heat shock protein 1, beta (HSP90AB1)	↑	↓	↓
35	Heat shock protein 5 precursor (GRP78)	↑	↓*	↓
38	Heat shock protein 90, beta (Grp94), member 1	↓	↓*	↓*
45	Keratin complex 2, basic, gene 8	↓	↑	↑*
47	Krt13 protein	↑	↑*	↓
54	Peroxiredoxin 2	↓	UN	↑
57	Proteasome alpha 1 subunit	↓	↓	↓
60	Protein synthesis initiation factor 4A	↑	↓	↓*
62	Put. beta-actin (aa 27-375)	↑	↑*	↑
68	Superoxide dismutase 1, soluble	↑	↑	↓
71	Tubulin, beta 5	↑	↓	↑
75	Valosin-containing protein	↓	↓*	↓*
76	Vimentin	↑	↓	↓*
	Total changes	11↓, 10↑	14↓, 6↑, 1UN	15↓, 6↑
	Total significant changes	1↓*	5↓, 4↑	6↓, 1↑

Comparison of mean normalized volumes (see Additional File 4) for proteins from KO mice to KO 6 hr SP-A, KO 18 hr SP-A, and wild-type mice. Increased (↑), decreased (↓), unchanged (UN), determined to be significant (p < 0.05) by t-test (*).

ISSN: 1477-5956