Table 1 Major proteins (>0.1 %) of the zebra finch eggshell calcified layer
From: The calcified eggshell matrix proteome of a songbird, the zebra finch (Taeniopygia guttata)
Protein | Accession | iBAQ % | iBAQ % in turkey 1 /quail 2 eggshell | Abundance in chicken eggshell 3 (emPAI) |
|---|---|---|---|---|
Ovalbumin(−like) | gi|224045100 | 35.20 | 12.52/15.97 | High (65.3)3a |
Similar to ovocleidin-116 | gi|224049274/H0YVI0 | 8.37 | 31.30/46.40 | High (65.3)3a |
Ovalbumin-related protein Y-like | gi|224045098 | 5.60 | 0.43/0.21 | Intermediate (3.8)3a |
Ovalbumin-like (OVALX?) | gi|224045096/H0YV21 | 4.08 | <0.01/0.21 | Intermediate (3.8)3a |
BPIL1/Tenp-like | H0Z7I9/gi|449486399 | 3.72 | 0.01/- | Low (0.6)3a |
Similar to serum albumin (ALB) | gi|449499399/H0YTL4 | 2.93 | 2.57/0.39 | High (113.5)3a |
Similar to ovomucoid | H0YQL9/gi|224067572 | 1.73 | 0.05/0.11 | Intermediate (2.2)3a |
gi|449512555/H1A0C8 | ||||
Similar to Pigment epithelium-derived factor (PEDF) | H0Z5S2/gi|449479753 | 1.62 | 0.64/0.31 | Intermediate (6.0)3a |
Similar to BPI fold-containing family B member 4/Ovocalyxin-36 | gi|224078167/H0Z7K8 | 1.59 | 6.00/7.80 | High (23.2)3a |
Insulin-like growth factor-binding protein 5 (IGFBP5) | H0Z3W8/gi|449506910 | 1.26 | -/0.13 | Low (0.6)3a |
Similar to vitellogenin-2 (VIT2) | gi|224057610/H0Z847 | 1.22 | 0.02/<0.01 | Low (1.7)3a |
Similar to ovostatin (A2M-1) | H0ZS62 | 1.20 | 0.05/0.08 | Low (1.5)3a |
Similar to vitellogenin-1 (TPRXL) | H0Z8U7/gi|224057648 | 1.08 | 0.01/0.01 | Low (0.8)3a |
Glutathione peroxidase 3 (GPX3) | gi|401664564/H0YRF2 | 1.06 | 0.04/0.36 | Intermediate (2.2)3a |
Ubiquitin (C) | B5G2Q3/gi|5822013 | 1.06 | 0.12/0.12 | High (9.0)3a |
Actin(s) | H0Z0Q3/gi|224061779 | 1.05 | -/0.36 | High (9.0)3a |
Putative apolipoprotein A-I | B5G356/gi|350538495 | 0.98 | 0.05/0.17 | Intermediate (2.2)3a |
Similar to mesothelin | gi|449476510/H0ZYM3 | 0.91 | <0.01/<0.01 | Low (0.4)3a |
Similar to Nucleobindin-2 (NUCB2) | H0ZEB8/gi|449501999 | 0.76 | 0.23/0.07 | Intermediate (7.3)3a |
Similar to leukocyte antigen 86 (LY86) | H0YVQ1/gi|224045162 | 0.72 | <0.01/<0.01 | Intermediate (0.3)3a |
Similar to ovotransferrin (LTF) | H0Z885/gi|449509524 | 0.69 | 2.22/0.19 | High (22.9)3a |
Similar to Sulfhydryl oxidase 1 (QSOX1) | H1A439/gi|449509315 | 0.56 | 1.08/0.18 | Intermediate (4.7)3a |
Similar to Mucin-5 AC | gi|449504198/H0ZGZ6/ | 0.54 | 0.15/0.35 | Low (1.4)3a |
H0ZGY9/H0ZGW8 | ||||
Similar to plasminogen (LPA-2) | gi|224048112/H0ZML2 | 0.53 | <0.01/- | - |
Similar to ovoinhibitor | gi|449474881/H0YQL7 | 0.53 | 0.01/0.01 | High (12.1)3a |
Similar to cathepsin D (CTSD) | H0ZGM2/gi|224050910 | 0.50 | 0.22/0.18 | Intermediate (5.3)3a |
Similar to multiple inositol polyphosphate phosphatase 1 (MINPP1) | gi|224052234/H0Z3R0 | 0.43 | <0.01/<0.01 | Low (2.1)3a |
Similar to ovocalyxin-32 (RARRES1) | H0ZLS5/gi|449510033 | 0.40 | −/− | High (71.0)3a |
Similar to Angiopoietin-related protein 3 (ANGPTL3) | gi|224058351/H0ZHW1 | 0.39 | −/− | Low (2.0)3a |
Fibronectin type III domain-containing protein 1 (FNDC1) | gi|449497556/H0ZMA1 | 0.39 | −/− | Intermediate3b |
Similar to tumor necrosis factor receptor superfamily member 6B (TNFRSF6B) | gi|224078299/H0Z9P3 | 0.38 | 0.01/0.01 | Intermediate3b |
Calcitonin gene-related peptide 2 (CALCB)/ procalcitonin | H0ZDU9/gi|224050809 | 0.37 | -/0.01 | - |
Similar to N-acetyl-glucosamine-6-sulfatase (GNS) | H0Z834/gi|449481578 | 0.36 | 0.04/0.03 | Low (0.8)3a |
Similar to Tetraspanin (TSPAN1) | gi|449508571/H0ZCW3 | 0.33 | −/− | - |
Similar to carbonic anhydrase 4(CA4) | H0ZCC0/gi|449480381 | 0.32 | 0.35/0.03 | Intermediate (2.9)3a |
Similar to avidin | H0YUD8/gi|224090240 | 0.30 | 2.67/1.95 | Intermediate (2.2)3a |
Similar to Lysyl oxidase 2 (LOXL2) | H1A3U9/gi|449488345 | 0.30 | 0.01/0.04 | Low (0.2)3a |
Prostatic acid phosphatase-like; domain: Histidine phosphatase superfamily | gi|449493197 | 0.29 | −/− | Low (1.6)3a |
Similar to alpha-2-HS-glycoprotein (AHSG) | gi|449509821/H0ZJJ6 | 0.29 | 0.01/- | Low (1.3)3a |
Similar to lactadherin (MFGE8) | H0ZD74/gi|449471648 | 0.27 | 1.25/0.36 | High (15.9)3a |
Similar to stanniocalcin-1(STC1) | H0Z285/gi|449488187 | 0.27 | 0.01/<0.01 | Low (0.5)3a |
Similar to 45 kDa calcium-binding protein (SDF4) | H0Z0U7/gi|224080053 | 0.26 | 0.09/0.03 | Intermediate (2.9)3a |
Similar to ovostatin | gi|449485099/H1A5U5 | 0.23 | 0.05/0.34 | Low (1.5)3a |
Putative cystatin | B5G368/gi|197129295 | 0.22 | 1.67/0.11 | High (45.4)3a |
Similar to heat shock cognate 71 kDa protein (HSPA8) | gi|224083318/H0YQE7 | 0.22 | 0.01/0.01 | Low (1.23)3a |
Putative peroxiredoxin 1 | B5G0M2/gi|197128341 | 0.21 | 0.01/0.02 | Low (1.0)3a |
Semaphorin-3 F | gi|224066001/H0Z7X2 | 0.21 | <0.01/- | - |
Similar to epididymis-specific alpha-mannosidase (MAN2B2) | H0ZIJ5/gi|449501297 | 0.21 | 0.06/0.02 | Low (0.8)3a |
Slit homolog 2 protein (SLIT2) | H0ZH52/gi|449500994 | 0.21 | 0.01/0.01 | Low (0.6)3a |
Similar to glia-derived nexin (SERPINE2) | gi|224060004/H0ZCA5 | 0.20 | 0.18/0.31 | High (11.1)3a |
Similar to proactivator polypeptide (PSAP-1) | H0Z157/gi|449504732 | 0.20 | 0.06/0.12 | High (9.0)3a |
Peptidyl-prolyl cis-trans isomerase B | B5G4N8/gi|350536587 | 0.19 | -/0.06 | High (13.7)3a |
Similar to lysosomal Pro-X carboxypeptidase (PRCP) | gi|224043680/H0ZRS7 | 0.19 | -/<0.01 | - |
Renin/prorenin receptor (ATP6AP2) | H0Z8C1/gi|449482762 | 0.19 | 0.05/0.03 | Low (1.8)3a |
Similar to cathepsin L1/L2 (CTSL1) | H0YQQ3/gi|449513868 | 0.18 | 0.02/0.02 | Low (0.2)3a |
Similar to apovitellenin-1 (APOVLDLII) | gi|224044051/H0ZT64 | 0.16 | 0.01/0.16 | Low (1.5)3a |
Alpha-enolase (ENO1) | H0Z0D8/gi|224079993 | 0.16 | 0.01/0.02 | Low (1.1)3a |
Similar to beta-hexosaminidase subunit beta (HEXB) | H0Z6E7/gi|224091413 | 0.16 | 0.03/<0.01 | Low (0.6)3a |
Similar to Netrin-3 (NTN3) | gi|449475443/H0YYI9 | 0.15 | −/− | - |
Similar to Carboxypeptidase (CTSA) | H0Z6V7/gi|449486379 | 0.15 | 0.08/0.02 | Intermediate (2.4)3a |
Histone H4 | B5FXC8/gi|350536313 | 0.14 | 0.04/0.03 | Low (1.2)3a |
EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1)/Fibulin-5 | H0Z947/gi|224047478 | 0.14 | 0.02/- | Low (0.2)3a |
Ezrin (EZR) | H0ZLB9/gi|449497375 | 0.14 | <0.01/0.02 | Low (1.2)3a |
Syntenin-1 (SDCBP) | H0ZLL3/gi|449494168 | 0.13 | 0.02/0.10 | Intermediate (2.8)3a |
Similar to hyaluronan and proteoglycan link protein 3 (HAPLN3) | H0ZD86/gi|449471652 | 0.13 | 0.05/0.07 | Low (1.2)3a |
Similar to angiotensinogen (AGT) | gi|224047800/H0ZI58 | 0.12 | <0.01/0.01 | Low (0.2)3a |
Similar to vitamin K-dependent protein S (PROS1) | H0ZTE6/gi|449485775 | 0.12 | 0.03/- | Low (0.4)3a |
L-lactate dehydrogenase | B5G2G3/gi|310703675 | 0.11 | 0.01/0.01 | Low (0.2)3a |
Similar to ceroid-lipofuscinosis neuronal protein 5 (CLN5) | H0ZQM7/gi|224043499 | 0.11 | 0.02/0.01 | Low (0.6)3a |
Similar to Riboflavin-binding protein | H0ZCK8/gi|224058069 | 0.11 | 0.02/0.01 | Low (0.7)3a |
Similar to Out at first protein homolog (OAF) | H0YQ63/gi|224083284 | 0.11 | <0.01/0.03 | Low (1.7)3a |
Similar to alpha-2-antiplasmin | gi|449480130/H0Z5Q3 | 0.11 | 0.60/0.80 | Intermediate3b |
Similar to solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) | gi|449484853/H0ZSC5 | 0.11 | -/0.01 | Low (1.8)3a |
Similar to nephronectin (NPNT) | H0Z095/gi|449500286 | 0.11 | 0.02/0.01 | Intermediate (3.8)3a |
Similar to alpha-2-macroglobulin-like 1 | H0ZSA0 | 0.11 | -/0.01 | Low (0.2)3a |