Fig. 4
From: Stathmin involvement in the maternal embryonic leucine zipper kinase pathway in glioblastoma
MELK and stathmin protein expression in non-neoplastic and astrocytoma tissue samples. Immunohistochemistry was performed for MELK a–f and stathmin (g–l) in 6 cases each of in (a and g) non-neoplastic (NN) brain tissues, (b and h) pilocytic (AGI), (c and i) low grade (AGII), (d and j) anaplastic (AGIII) astrocytomas, and (e and k) glioblastoma (GBM). Pictures show representative cases of each sample type, with 400-x magnification. The bar (k) represent the scale bar of 10 μm. The graphs (f and l) represent a semi-quantitative immunolabelling score (ILS) calculated as the product of staining intensity and the percentage of positive cells. Both MELK and stathmin showed positive staining in the cytosol. MELK reactivity increased with the increment of the malignancy among diffusely infiltrating astrocytomas (AGII-AGIV). Stathmin cytosol staining was stronger in GBM cases. The horizontal bars show the median ILS of each group