Fig. 2From: Identification of different proteins binding to Na, K-ATPase α1 in LPS-induced ARDS cell model by proteomic analysisProcedure summary of Co-IP-MS techniques. Using this approach with IP-level antibodies are available against the target protein. For untransfected sample, protein complex is affinity captured from native cell lysates by an immobilized antibody that specifically recognizes an epitope of target (bait) protein. The co-isolated protein complex is washed extensively to remove unspecifically bound proteins and is subsequently eluted from the resin prior to protein identification by mass spectrometryBack to article page