Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus

Stephani, Laurentia; Rahayu, Puji; Retnoningrum, Debbie; Suhartono, Maggy Thenawidjaja; Rachmawati, Heni; Tjandrawinata, Raymond R.

doi:10.1186/s12953-023-00206-9

Proteome Science

Table 2 Peptide information of F25 and F85 fraction generated from MS spectra

From: Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus

Spot	Protein hit	Matched peptide	Protein score	Peptide	Peptide position	Identity	Mass deviation (%)	Ion score
P2	Annexin	3	84	KSALSGHLETVILGLLKT	88–98	100	0.0051	39
				KELSAALKSALSGHLETVILGLLKT	81–105	100	-0.0103	6
				KSALSGHLETVILGLLKTPAQYDASELKA	88–116	100	0.0719	39
P5	Fibrinolytic protease 1	2	60	RTGSSNVLPDTLQKA	137–151	100	0.0070	60
P5	Fibrinolytic protease 1	2	60	KSIVHPSYNSNTLNNDIMLIKL	83–97	53	-0.0027	60
P7	Lumbrokinase, partial	4	130	RTDGTNNLPDILQKS	130–143	64	-0.0047	73
				VIGGTNASPGEFPWQLSQQRQ	1–19	89	0.0000	57
				RTSAYLSWIANNS	223–235	100	0.0012	34
				RTGSSNVLPDTLQKA	130–144	100	0.0041	78
P8	Fibrinolytic protease 1	3	110	RTSAYLSWIANNS	230–242	100	-0.0031	32
				RTGSSNVLPDTLQKA	137–151	100	-0.0145	79
				RTDGTNNLPDILQKS	137–150	64	0.0028	71
P9	Fibrinolytic protease 1	4	130	RTSAYLSWIANNS	230–242	100	0.0028	36
				RTGSSNVLPDTLQKA	137–151	100	0.0034	79
				RGGSHSCGASLLNALNGLSASHCVDGAAPGTI	26–57	100	-0.0235	15
				RTDGTNNLPDILQKS	137–150	64	0.0051	77
D1	Lumbrokinase	11	202	RTLENDVSVIKT	86–97	100	-0.0614	51
				RVGFHAGWITDTITNN	223–238	100	-0.0859	53
				RQTHDVDSIFVNENYDPRT	69–87	100	-0.1096	65
				KIVGGIEARPYEFPWQVSVRR	8–28	100	-0.1177	33
				RTSSSNILPDTLQKA	131–145	100	-0.0773	76
				VIGGTDAAPGEFPWQLSQTRG	1–21	100	-0.1139	63
				RTSAYLDWIAANSS	225–236	100	-0.0770	31
				RVGSQTGWITDIITNN	224–239	100	-0.0873	37
				RQTHDVDSIFVHEDYNGNTFENDVSVIKT	69–97	100	-0.1646	26
				PYEFPWQVSVRRK	9–21	100	-0.0882	55
				RTGSSNVLPDTLQKA	130–144	100	-0.1090	79
D2	Lumbrokinase partial	3	141	RTSSSNILPDTLQKA	131–145	100	-0.0804	67
				VIGGTDAAPGEFPWQLSQTRG	1–21	100	-0.1159	74
				RTSAYLDWIAANSS	225–236	100	-0.0769	35
D3	Lumbrokinase partial	3	128	RTSSSNILPDTLQKA	131–145	100	-0.0817	66
				VIGGTDAAPGEFPWQLSQTRG	1–21	100	-0.1177	62
				RTSAYLDWIAANSS	225–236	100	-0.0752	30
D4	Lumbrokinase	10	184	RTLENDVSVIKT	86–97	100	-0.0624	53
				RVGFHAGWITDTITNN	223–238	100	-0.0872	47
				RQTHDVDSIFVNENYDPRT	69–87	100	-0.1169	45
				KIVGGIEARPYEFPWQVSVRR	8–28	100	-0.1244	39
				RTSSSNILPDTLQKA	131–145	100	-0.0780	67
				VIGGTDAAPGEFPWQLSQTRG	1–21	100	-0.1176	58
				RTSAYLDWIAANSS	225–236	100	-0.0820	20
				RVGSQTGWITDIITNN	224–239	100	-0.0955	24
				PYEFPWQVSVRRK	9–21	100	-0.0766	52
				RTGSSNVLPDTLQKA	130–144	100	-0.0794	52
D5	Lumbrokinase	3	81	RTSSSNILPDTLQKA	131–145	88	-0.0136	68
				RVGFHAAWITDIITNN	230–245		+ 0.0126	49
				RVGSQTGWITDIITNN	224–239	100	-0.0178	35

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