Fig. 1

Age-related proteomic changes in neonatal C57BL/6J mouse hearts from day 1 to day 7. A Workflow of the quantitative proteomic analysis of neonatal mouse hearts; (B) and (C) Gaussian distribution of dimethyl labeling ratio. The “significantly changed” threshold was set by μ ± 1.96σ. The “significantly changed” ratios between the P4/P1 groups were > 2.18 or < 0.46, and between the P7/P1 groups > 2.27 or < 0.43; (D) Heatmap of significantly changed proteins. Red and blue reflect high and low expression, respectively; (E) Real-time PCR results of selected genes (n = 3). (F) Western blotting of selected genes (n = 3)