Fig. 4

Inhibition of PKA promoted EdU-DNA incorporation in cardiomyocytes. A, B EdU-DNA incorporation assay in primary cardiomyocytes treated with DMSO and the PKA activator (1μM, 5μM, 10μM bucladesine) or the PKA inhibitor (10nM, 100nM, 1 μM H-89). EdU staining (red fluorescence), DAPI staining (blue fluorescence), scale bar = 20 μm; C, D Statistical analysis of EdU results. 5μM and 10μM bucladesine can inhibit cardiomyocyte replication (from 13.03% to 7.13% p = 0.0023 and 7.78% p = 0.0102) and 1μM H-89 can promote cardiomyocyte replication (from 12.26% to 19.25%, p = 0.0004)