Fig. 2

GO, KEGG analyses and protein-protein interaction (PPI) network construction of DEPs. a GO analysis of differentially expressed proteins in Niangya yak sperm (biological process, cell component, molecular function). b Scatter map of KEGG pathway enrichment of differentially expressed proteins. (Circle: pathway. Abscissa: enrichment factor. Ordinate: pathway name. Enrichment factor: the ratio of the proportion of annotated differentially expressed proteins in a protein pathway to the proportion of all annotated proteins in that pathway. Enrichment factor size: the level at which a protein is enriched in the pathway; the larger the enrichment factor, the more significant the enrichment level of differentially expressed proteins in this pathway. Color of the circle: qvalue, where qvalue is the P value after correction for multiple hypothesis testing, and a smaller qvalue indicates a more reliable enrichment significance of the differentially expressed proteins in this pathway. Circle size: the number of proteins enriched within the pathway, with larger circles indicating more proteins. This figure is from www.kegg.jp/kegg/kegg1.html) c PPI network of the DEPs involved in important pathways. (The nodes are proteins, and the edges are interactions)