Fig. 4

Comparative analysis of primary tumors-derived exosomal proteins between CHMp and SW480. A Venn diagram illustrating the 19 common proteins between CHMp and SW480 DEPs. Venn diagram visualized using the Venny 2.1. DEPs satisfying the fold change > 1.2 and p-value < 0.05. B The five proteins commonly enriched in both CHMp and SW480 exosomal proteins. (Purple bars; CHMp enriched, yellow bars; SW480 enriched). GPI showed highly enriched in both CHMp and SW480. C Glycolysis and gluconeogenesis pathway. Primary tumor enriched enzymes (GPI, LDHA, LDHB, TPI1, and ALDOA) were bolded. D Western blot analysis of primary tumor and metastases-derived exosomes for GPI and LDHA. GPI showed enrichment both in CHMp and SW480, whereas LDHA showed enrichment only in CHMp exosomes. Alix was used as loading control. E Kaplan–Meier survival curves of GPI, LDHA, TPI1, and ALDOA of breast cancer and colorectal cancer patients. High-level expressions of GPI, LDHA, TPI1 and ALDOA in breast and colorectal cancer patients showed worse overall survival outcomes. Kaplan–Meier plots derived from http://kmplot.com/analysis/