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Figure 1 | Proteome Science

Figure 1

From: MALDI/MS peptide mass fingerprinting for proteome analysis: identification of hydrophobic proteins attached to eucaryote keratinocyte cytoplasmic membrane using different matrices in concert

Figure 1

MALDI/MS spectra obtained with four different matrices, for the sample located at 53 kDa on the 1D gel. The samples were prepared, via the dried droplet method, by mixing 0.7 μl of sample with 0.7 μl of: (a) 60 μg/μl DHB solution in methanol (laser intensity 3000 kW.cm-2), (b) a mixture of three matrices: 50/45/5 (v/v/v) [20 μg/μl succinic acid in 1% formic acid] / [25 μg/μl DHB in 30/50 (v/v) CH3CN/1% formic acid] / [25 μg/μl DHBs in 30/50 (v/v) CH3CN/1% formic acid] (laser intensity 3300 kW.cm-2), (c) 10 μg/μl HCCA solution in methanol (laser intensity 2700 kW.cm-2), or (d) 10 μg/μl SA (sinapinic acid) solution in 30/50 (v/v) CH3CN/1% formic acid (laser intensity 3000 kW.cm-2).

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