Table 1 Proteins identified in the human cerebral microdialysate. The upper part contains proteins also found in CSF, the lower part lists additional proteins found in the microdialysate which have not been described in CNS. The first column gives the consecutive number of the identified protein, the second column the number of spots in the two-dimensional gels belonging to the individual proteins. The third and fourth column displays the protein annotation as in the SwissProt database at http://www.expasy.ch. The amino acid composition or structural information about the proteins can be retrieved via the accession number in the fifth column. This database also contains the CSF protein map. The sixth and seventh column shows pI and molecular weight. Strictly speaking, the immunoglobulin fractions do not represent a single protein, but a protein family, therefore pI and molecular weight cannot be given.
Spot number | Number of spots identified | Gene name | Protein description | SwissProt accession number | Theoretical pI | Theoretical molecular weight (Da) |
|---|---|---|---|---|---|---|
proteins identified in cerebral microdialysate and cerebrospinal fluid | ||||||
1 | 8 | SERPINA3 | Alpha-1-antichymotrypsin | P01011 | 5.33 | 47651 |
2 | 8 | SERPINA1 | Alpha-1-antitrypsin | P01009 | 5.37 | 46737 |
3 | 5 | A1BG | Alpha-1B-glycoprotein | P04217 | 5.65 | 51941 |
4 | 3 | APOA1 | Apolipoprotein A-I | P02647 | 5.56 | 30778 |
5 | 1 | AMBP | AMBP protein | P02760 | 5.95 | 38999 |
6 | 2 | APOE | Apolipoprotein E | P02649 | 5.65 | 36154 |
7 | 2 | C3 | Complement C3 | P01024 | 6.02 | 187164 |
8 | 1 | FGG | Fibrinogen gamma chain | P02679 | 5.37 | 51512 |
9a | 6 | HP | Haptoglobin-2 | P00738 | 6.13 | 45205 |
9b | 2 | HP | Haptoglobin alpha-2 chain | P00738 | 5.57 | 15946 |
9c | 2 | HP | Haptoglobin beta chain | P00738 | 6.32 | 27265 |
10 | 7 | -- | Immunoglobulin heavy chain | P99006 | -- | -- |
11 | 13 | -- | Immunoglobulin light chain | P99007 | -- | -- |
12 | 1 | RBP4 | Plasma retinol-binding protein | P02753 | 5.76 | 23044 |
13 | 4 | PLG | Plasminogen (EC 3.4.21.7) | P00747 | 7.04 | 90569 |
14 | 5 | ALB | Albumin | P02768 | 5.92 | 69367 |
15 | 5 | TF | Transferrin | P02787 | 6.81 | 77050 |
16 | 2 | TTR | Transthyretin | P02766 | 5.52 | 15887 |
17 | 2 | GC | Vitamin D-binding protein | P02774 | 5.24 | 56761 |
proteins identified solely in cerebral microdialysate | ||||||
18 | 1 | B2M | Beta-2 microglobulin | P01887 | 7.80 | 13823 |
19 | 1 | CKB | Creatine kinase, B chain | P12277 | 5.34 | 43644 |
20 | 1 | DRP2 | Dihydropyrimidinase related protein-2 | Q16555 | 5.95 | 62294 |
21 | 4 | GAPD | Glyceraldehyde 3-phosphate dehydrogenase | P16858 | 8.45 | 35679 |
22 | 1 | HSP73 | Heat shock protein 70 kD HSP73 | P11142 | 5.37 | 70898 |
23 | 1 | HBB | Hemoglobin beta chain | P02023 | 6.81 | 15867 |
24 | 1 | MDHA | Malate dehydrogenase | P40925 | 6.89 | 36295 |
25 | 4 | TUBA1 | Tubulin alpha-1 | P04687 | 4.94 | 50152 |
26 | 3 | -- | Tubulin beta | Q13885 | 4.78 | 49907 |
27 | 1 | TUBB5 | Tubulin beta 5 | Q8WUC1 | 4.78 | 49671 |